Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22306 | 5' | -59.8 | NC_005045.1 | + | 6188 | 0.66 | 0.384779 |
Target: 5'- aGGCCgaGGCucGUGAGCgggcgaagGCGgCGGCUa -3' miRNA: 3'- -CUGGa-CCGcaUACUCGa-------CGCgGCCGA- -5' |
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22306 | 5' | -59.8 | NC_005045.1 | + | 40595 | 0.67 | 0.342545 |
Target: 5'- aGGCC-GGCGagggcagccgccaGGGCUGCGCCGcGCa -3' miRNA: 3'- -CUGGaCCGCaua----------CUCGACGCGGC-CGa -5' |
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22306 | 5' | -59.8 | NC_005045.1 | + | 10266 | 0.67 | 0.332725 |
Target: 5'- cGACCgGGCGUAUGGcccgcaagaccccGCUGaccaagGCCGaGCUg -3' miRNA: 3'- -CUGGaCCGCAUACU-------------CGACg-----CGGC-CGA- -5' |
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22306 | 5' | -59.8 | NC_005045.1 | + | 7959 | 0.67 | 0.325497 |
Target: 5'- cGACCcggcaGGUGUccgugguaGUGAGgcgugaUGCGCCGGCUc -3' miRNA: 3'- -CUGGa----CCGCA--------UACUCg-----ACGCGGCCGA- -5' |
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22306 | 5' | -59.8 | NC_005045.1 | + | 20908 | 0.67 | 0.305274 |
Target: 5'- aGGCC-GGCGccccauccgaacugAUGGGcCUGUGCUGGCUg -3' miRNA: 3'- -CUGGaCCGCa-------------UACUC-GACGCGGCCGA- -5' |
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22306 | 5' | -59.8 | NC_005045.1 | + | 611 | 0.67 | 0.30225 |
Target: 5'- -cCCUGGCGcUGUGcuuguGCUGCcGCUGGUc -3' miRNA: 3'- cuGGACCGC-AUACu----CGACG-CGGCCGa -5' |
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22306 | 5' | -59.8 | NC_005045.1 | + | 32122 | 0.67 | 0.30225 |
Target: 5'- cGugCUGGC--AUG-GCUGCuGCUGGCg -3' miRNA: 3'- -CugGACCGcaUACuCGACG-CGGCCGa -5' |
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22306 | 5' | -59.8 | NC_005045.1 | + | 2931 | 0.7 | 0.205071 |
Target: 5'- -uCCUGGCGgguucGUGguAGUggGCGCCGGCg -3' miRNA: 3'- cuGGACCGCa----UAC--UCGa-CGCGGCCGa -5' |
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22306 | 5' | -59.8 | NC_005045.1 | + | 18899 | 0.71 | 0.174449 |
Target: 5'- -uCCUGGCccagGUGgcgGAGCUGCGCCuGCc -3' miRNA: 3'- cuGGACCG----CAUa--CUCGACGCGGcCGa -5' |
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22306 | 5' | -59.8 | NC_005045.1 | + | 8451 | 1.08 | 0.000261 |
Target: 5'- cGACCUGGCGUAUGAGCUGCGCCGGCUg -3' miRNA: 3'- -CUGGACCGCAUACUCGACGCGGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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