Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22309 | 3' | -57.4 | NC_005045.1 | + | 284 | 0.66 | 0.497102 |
Target: 5'- aGCCAGGGCCaGAGCcaggGAAGaccguagGCUgGCGc -3' miRNA: 3'- -UGGUUCCGG-CUCGa---CUUCa------CGGaCGU- -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 42347 | 0.66 | 0.497102 |
Target: 5'- aGCCAGGGCCaGAGCcaggGAAGaccguagGCUgGCGc -3' miRNA: 3'- -UGGUUCCGG-CUCGa---CUUCa------CGGaCGU- -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 6959 | 0.66 | 0.497102 |
Target: 5'- -aCGGGccGCCGAGCUGGcuGGUGCCg--- -3' miRNA: 3'- ugGUUC--CGGCUCGACU--UCACGGacgu -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 4061 | 0.66 | 0.497102 |
Target: 5'- uGCCucGGCCu-GCUGGcaGGcGCCUGCu -3' miRNA: 3'- -UGGuuCCGGcuCGACU--UCaCGGACGu -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 41169 | 0.66 | 0.486564 |
Target: 5'- gACCAGaaccGCCcguGGGCUGAccggccuGUGCCUGCu -3' miRNA: 3'- -UGGUUc---CGG---CUCGACUu------CACGGACGu -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 15852 | 0.66 | 0.486564 |
Target: 5'- gGCgGAGGCCGAGgUGcuGUGgaUGCGc -3' miRNA: 3'- -UGgUUCCGGCUCgACuuCACggACGU- -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 23837 | 0.66 | 0.485516 |
Target: 5'- cACCAAGGCCGccaucggucGGCUGAuggacucGGgcgaugcccagGCCaUGCAa -3' miRNA: 3'- -UGGUUCCGGC---------UCGACU-------UCa----------CGG-ACGU- -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 14123 | 0.66 | 0.476132 |
Target: 5'- aGCCuuguuGGCCaGGGC-GAGGgcgGCUUGCAg -3' miRNA: 3'- -UGGuu---CCGG-CUCGaCUUCa--CGGACGU- -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 40826 | 0.66 | 0.465813 |
Target: 5'- aACCAAGGCCGcGCcacUGAGGgcUGCCaaGCc -3' miRNA: 3'- -UGGUUCCGGCuCG---ACUUC--ACGGa-CGu -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 13275 | 0.66 | 0.44553 |
Target: 5'- uGCCAuccGGCCccaucucGCUGAAGgcGCCUGCc -3' miRNA: 3'- -UGGUu--CCGGcu-----CGACUUCa-CGGACGu -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 14966 | 0.68 | 0.37868 |
Target: 5'- cGCUggGaGCCGu-CUGAGGcacUGCCUGCGg -3' miRNA: 3'- -UGGuuC-CGGCucGACUUC---ACGGACGU- -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 27429 | 0.68 | 0.343623 |
Target: 5'- uGCCGGGGCCG-GCUGAguacauccaggcGGcgGCCUccaGCGg -3' miRNA: 3'- -UGGUUCCGGCuCGACU------------UCa-CGGA---CGU- -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 14804 | 0.69 | 0.318902 |
Target: 5'- -gCAGGGCCGAGCcgggGAAGUuggugGCCgaGCGg -3' miRNA: 3'- ugGUUCCGGCUCGa---CUUCA-----CGGa-CGU- -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 30100 | 0.69 | 0.310964 |
Target: 5'- cCCAggcgcAGGCCGAGCUGcguacCCUGCAc -3' miRNA: 3'- uGGU-----UCCGGCUCGACuucacGGACGU- -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 34476 | 0.69 | 0.303177 |
Target: 5'- -aCGGGGCCGAGCU--GGUGCUcacgcgGCAc -3' miRNA: 3'- ugGUUCCGGCUCGAcuUCACGGa-----CGU- -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 36223 | 0.72 | 0.209045 |
Target: 5'- cGCCAGGGCCGGccacgauGUUGAGGUcgcgaaugcccGCCUGUu -3' miRNA: 3'- -UGGUUCCGGCU-------CGACUUCA-----------CGGACGu -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 11929 | 0.77 | 0.086981 |
Target: 5'- aAUCGAGGCCGAGCUGAAGaaGCUgaUGCc -3' miRNA: 3'- -UGGUUCCGGCUCGACUUCa-CGG--ACGu -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 23471 | 0.84 | 0.02558 |
Target: 5'- uACCGGGGCCG-GCUGAgcgGGUGCUUGCGg -3' miRNA: 3'- -UGGUUCCGGCuCGACU---UCACGGACGU- -5' |
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22309 | 3' | -57.4 | NC_005045.1 | + | 10297 | 1.08 | 0.000414 |
Target: 5'- gACCAAGGCCGAGCUGAAGUGCCUGCAc -3' miRNA: 3'- -UGGUUCCGGCUCGACUUCACGGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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