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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22309 | 5' | -57 | NC_005045.1 | + | 27819 | 0.69 | 0.339755 |
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Target: 5'- -cCGGCGCCuacauggagcguACCCAUcUCGGUGUGa- -3' miRNA: 3'- aaGCUGCGG------------UGGGUAuAGCCGCACga -5' |
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| 22309 | 5' | -57 | NC_005045.1 | + | 15547 | 0.69 | 0.338921 |
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Target: 5'- -aUGACGauCUACCCGUAUugggagaucgacaCGGCGUGCg -3' miRNA: 3'- aaGCUGC--GGUGGGUAUA-------------GCCGCACGa -5' |
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| 22309 | 5' | -57 | NC_005045.1 | + | 14827 | 0.69 | 0.33148 |
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Target: 5'- cUCGcCGCCACCCAgca-GGaUGUGCa -3' miRNA: 3'- aAGCuGCGGUGGGUauagCC-GCACGa -5' |
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| 22309 | 5' | -57 | NC_005045.1 | + | 35941 | 0.71 | 0.256913 |
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Target: 5'- cUCGACGCCGCCaauga-GGCGcUGCg -3' miRNA: 3'- aAGCUGCGGUGGguauagCCGC-ACGa -5' |
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| 22309 | 5' | -57 | NC_005045.1 | + | 32091 | 0.72 | 0.219125 |
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Target: 5'- uUUCGGCGCUGgCCGUGcucugcggcUCGGuCGUGCUg -3' miRNA: 3'- -AAGCUGCGGUgGGUAU---------AGCC-GCACGA- -5' |
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| 22309 | 5' | -57 | NC_005045.1 | + | 39293 | 0.73 | 0.18114 |
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Target: 5'- cUCGACGgCACCCGUGgcUgGGCG-GCUg -3' miRNA: 3'- aAGCUGCgGUGGGUAU--AgCCGCaCGA- -5' |
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| 22309 | 5' | -57 | NC_005045.1 | + | 2329 | 0.76 | 0.115513 |
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Target: 5'- aUCGACGCCAUCCGUGUCcGUG-GCa -3' miRNA: 3'- aAGCUGCGGUGGGUAUAGcCGCaCGa -5' |
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| 22309 | 5' | -57 | NC_005045.1 | + | 10333 | 1.05 | 0.000856 |
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Target: 5'- aUUCGACGCCACCCAUAUCGGCGUGCUc -3' miRNA: 3'- -AAGCUGCGGUGGGUAUAGCCGCACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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