miRNA display CGI


Results 1 - 12 of 12 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22310 3' -54.5 NC_005045.1 + 10409 1.1 0.00069
Target:  5'- gUCCGCAGCCUCAUGGAAGAUUGCCGGc -3'
miRNA:   3'- -AGGCGUCGGAGUACCUUCUAACGGCC- -5'
22310 3' -54.5 NC_005045.1 + 28485 0.71 0.39254
Target:  5'- gCCGUGGCCUCGcaggUGGAggccaagcgccaggGGAUgcuaGCCGGc -3'
miRNA:   3'- aGGCGUCGGAGU----ACCU--------------UCUAa---CGGCC- -5'
22310 3' -54.5 NC_005045.1 + 21611 0.68 0.53826
Target:  5'- aCCGCAGCaaugCuGUGGGAGAagcUGCgGGa -3'
miRNA:   3'- aGGCGUCGga--G-UACCUUCUa--ACGgCC- -5'
22310 3' -54.5 NC_005045.1 + 29468 0.68 0.549237
Target:  5'- cCCgGCAG-CUCAgccUGGAAGAg-GCCGGc -3'
miRNA:   3'- aGG-CGUCgGAGU---ACCUUCUaaCGGCC- -5'
22310 3' -54.5 NC_005045.1 + 33104 0.68 0.549237
Target:  5'- gUCC-C-GCUUCGUGGAGGGUauccgcagccUGCUGGg -3'
miRNA:   3'- -AGGcGuCGGAGUACCUUCUA----------ACGGCC- -5'
22310 3' -54.5 NC_005045.1 + 30291 0.68 0.549237
Target:  5'- cCUGCucGGCCUCcgGGAuaccuGAUagcaucacguUGCCGGc -3'
miRNA:   3'- aGGCG--UCGGAGuaCCUu----CUA----------ACGGCC- -5'
22310 3' -54.5 NC_005045.1 + 14381 0.68 0.582544
Target:  5'- gCCGuCGGCCUCGuaagugUGGAacAGGUcGuCCGGg -3'
miRNA:   3'- aGGC-GUCGGAGU------ACCU--UCUAaC-GGCC- -5'
22310 3' -54.5 NC_005045.1 + 42116 0.67 0.593745
Target:  5'- aCUGCcuAGCCUCcUGGGAGcUUccggcuucGCCGGg -3'
miRNA:   3'- aGGCG--UCGGAGuACCUUCuAA--------CGGCC- -5'
22310 3' -54.5 NC_005045.1 + 32182 0.67 0.593745
Target:  5'- gCCGCaggGGCCgaCGUGGA----UGCCGGa -3'
miRNA:   3'- aGGCG---UCGGa-GUACCUucuaACGGCC- -5'
22310 3' -54.5 NC_005045.1 + 53 0.67 0.593745
Target:  5'- aCUGCcuAGCCUCcUGGGAGcUUccggcuucGCCGGg -3'
miRNA:   3'- aGGCG--UCGGAGuACCUUCuAA--------CGGCC- -5'
22310 3' -54.5 NC_005045.1 + 19510 0.67 0.638791
Target:  5'- aCU-CGGCCUCggGGAAGAgacCCGGa -3'
miRNA:   3'- aGGcGUCGGAGuaCCUUCUaacGGCC- -5'
22310 3' -54.5 NC_005045.1 + 14211 0.66 0.694865
Target:  5'- --aGCGGCC-CAUGGcGGugcccGCCGGg -3'
miRNA:   3'- aggCGUCGGaGUACCuUCuaa--CGGCC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.