Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22312 | 3' | -59.6 | NC_005045.1 | + | 24362 | 0.66 | 0.409928 |
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Target: 5'- uGCCGGUgaaGAGCu-GGAGCGCAgCCGa -3' miRNA: 3'- -UGGCCGga-CUCGcuCCUCGUGUaGGU- -5' |
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| 22312 | 3' | -59.6 | NC_005045.1 | + | 42003 | 0.66 | 0.400684 |
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Target: 5'- gGCUGGUgUGGGCG-GGAGUaguuacuccGgGUCCAa -3' miRNA: 3'- -UGGCCGgACUCGCuCCUCG---------UgUAGGU- -5' |
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| 22312 | 3' | -59.6 | NC_005045.1 | + | 8782 | 0.66 | 0.380823 |
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Target: 5'- gGCCGGCCUGGGUcgaaguaccgcuuGAGcugcGGCGCAauguguacggcgaUCCAg -3' miRNA: 3'- -UGGCCGGACUCG-------------CUCc---UCGUGU-------------AGGU- -5' |
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| 22312 | 3' | -59.6 | NC_005045.1 | + | 17834 | 0.67 | 0.315877 |
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Target: 5'- -gUGGcCCUGAcCGAGGAgGCugGUCCGc -3' miRNA: 3'- ugGCC-GGACUcGCUCCU-CGugUAGGU- -5' |
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| 22312 | 3' | -59.6 | NC_005045.1 | + | 35364 | 0.68 | 0.278836 |
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Target: 5'- cCCGGUCUucGGGCuGGcGGAGCAC-UCCAa -3' miRNA: 3'- uGGCCGGA--CUCG-CU-CCUCGUGuAGGU- -5' |
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| 22312 | 3' | -59.6 | NC_005045.1 | + | 28597 | 0.69 | 0.251758 |
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Target: 5'- cCCGGCCUGAGCcuc-GGCGCuUCCGc -3' miRNA: 3'- uGGCCGGACUCGcuccUCGUGuAGGU- -5' |
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| 22312 | 3' | -59.6 | NC_005045.1 | + | 3976 | 0.71 | 0.193502 |
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Target: 5'- cGCC-GCCUGGGCguGAGGAGUACGUaCAa -3' miRNA: 3'- -UGGcCGGACUCG--CUCCUCGUGUAgGU- -5' |
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| 22312 | 3' | -59.6 | NC_005045.1 | + | 10726 | 1.08 | 0.00029 |
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Target: 5'- cACCGGCCUGAGCGAGGAGCACAUCCAg -3' miRNA: 3'- -UGGCCGGACUCGCUCCUCGUGUAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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