Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22313 | 3' | -57.2 | NC_005045.1 | + | 21774 | 0.66 | 0.511152 |
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Target: 5'- aCCUCgcUGCCAagCUGGCg-CGGUCGCUg -3' miRNA: 3'- -GGAG--GCGGUagGGUCGaaGUCAGCGA- -5' |
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| 22313 | 3' | -57.2 | NC_005045.1 | + | 23082 | 0.67 | 0.47997 |
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Target: 5'- gCUCCGCCucugCCUguAGCUcgUCGG-CGCUc -3' miRNA: 3'- gGAGGCGGua--GGG--UCGA--AGUCaGCGA- -5' |
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| 22313 | 3' | -57.2 | NC_005045.1 | + | 652 | 0.68 | 0.401817 |
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Target: 5'- gCCU-CGCCAcUCCCucgccuGC-UCGGUCGCa -3' miRNA: 3'- -GGAgGCGGU-AGGGu-----CGaAGUCAGCGa -5' |
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| 22313 | 3' | -57.2 | NC_005045.1 | + | 18799 | 0.69 | 0.340436 |
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Target: 5'- -aUCCGCCAUCgcaCCAGUcgUUCcagGGUCGCg -3' miRNA: 3'- ggAGGCGGUAG---GGUCG--AAG---UCAGCGa -5' |
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| 22313 | 3' | -57.2 | NC_005045.1 | + | 1606 | 0.7 | 0.293456 |
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Target: 5'- aUCggCCGCCGauacuaCCCAGCauacugUCAGUCGCg -3' miRNA: 3'- -GGa-GGCGGUa-----GGGUCGa-----AGUCAGCGa -5' |
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| 22313 | 3' | -57.2 | NC_005045.1 | + | 10851 | 1.09 | 0.000474 |
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Target: 5'- cCCUCCGCCAUCCCAGCUUCAGUCGCUu -3' miRNA: 3'- -GGAGGCGGUAGGGUCGAAGUCAGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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