Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22313 | 5' | -47.1 | NC_005045.1 | + | 26690 | 0.67 | 0.965942 |
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Target: 5'- gCCGGccuuGCugCCGGGAGUCGGugccccuGGUGUGGg -3' miRNA: 3'- -GGUCau--UG--GGUCCUCAGUU-------UCAUACU- -5' |
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| 22313 | 5' | -47.1 | NC_005045.1 | + | 21713 | 0.67 | 0.95837 |
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Target: 5'- uCCGGUAGCCgGGGuGUCGuAGa---- -3' miRNA: 3'- -GGUCAUUGGgUCCuCAGUuUCauacu -5' |
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| 22313 | 5' | -47.1 | NC_005045.1 | + | 3238 | 0.7 | 0.867951 |
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Target: 5'- gCAGgcACCCGuGGAaucGUCGAGGcUGUGAc -3' miRNA: 3'- gGUCauUGGGU-CCU---CAGUUUC-AUACU- -5' |
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| 22313 | 5' | -47.1 | NC_005045.1 | + | 19429 | 0.71 | 0.821519 |
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Target: 5'- cCCAGgcuGCCCGGucGUCGAAGUAc-- -3' miRNA: 3'- -GGUCau-UGGGUCcuCAGUUUCAUacu -5' |
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| 22313 | 5' | -47.1 | NC_005045.1 | + | 39017 | 0.72 | 0.801249 |
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Target: 5'- cCCGGUAGCCCuGGAGggCGGcg-GUGAu -3' miRNA: 3'- -GGUCAUUGGGuCCUCa-GUUucaUACU- -5' |
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| 22313 | 5' | -47.1 | NC_005045.1 | + | 13933 | 0.73 | 0.735954 |
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Target: 5'- --uGUAGCCCAGG-GUCGccAGUAUGGu -3' miRNA: 3'- gguCAUUGGGUCCuCAGUu-UCAUACU- -5' |
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| 22313 | 5' | -47.1 | NC_005045.1 | + | 10889 | 1.13 | 0.003108 |
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Target: 5'- gCCAGUAACCCAGGAGUCAAAGUAUGAg -3' miRNA: 3'- -GGUCAUUGGGUCCUCAGUUUCAUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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