Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22314 | 3' | -55 | NC_005045.1 | + | 42088 | 0.66 | 0.673119 |
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Target: 5'- uCCCCCGUAG--GCccUCGUCuggugGGCCa -3' miRNA: 3'- -GGGGGCGUUgaUGu-AGCAGua---CCGGc -5' |
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| 22314 | 3' | -55 | NC_005045.1 | + | 25 | 0.66 | 0.673119 |
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Target: 5'- uCCCCCGUAG--GCccUCGUCuggugGGCCa -3' miRNA: 3'- -GGGGGCGUUgaUGu-AGCAGua---CCGGc -5' |
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| 22314 | 3' | -55 | NC_005045.1 | + | 27788 | 0.66 | 0.673119 |
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Target: 5'- gCCUCUGCcguguaucAGCUACAgcc-CGUGGCCGg -3' miRNA: 3'- -GGGGGCG--------UUGAUGUagcaGUACCGGC- -5' |
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| 22314 | 3' | -55 | NC_005045.1 | + | 33067 | 0.66 | 0.684161 |
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Target: 5'- aCCgCCUGC-GCUACAgCGaCGgugGGCCGg -3' miRNA: 3'- -GG-GGGCGuUGAUGUaGCaGUa--CCGGC- -5' |
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| 22314 | 3' | -55 | NC_005045.1 | + | 4080 | 0.66 | 0.695152 |
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Target: 5'- gCgCCUGCuACUGCAUCGcaGUGGCg- -3' miRNA: 3'- -GgGGGCGuUGAUGUAGCagUACCGgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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