miRNA: 3'- uGCUUCACUGgCuGGU--CCCACUC-ACc -5'
miRNA: 3'- -UGcUUCACUGGCu--GGuCCCACuCACC- -5'
miRNA: 3'- uGCUUCACUGGC--UGGucCCa--CUCACC- -5'
miRNA: 3'- -UGCUucacuGGCuGGUCCCACUCaCC- -5'
miRNA: 3'- -UGCUUCACUGGCUGGUCCCACUCACC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22315 | 3' | -55 | NC_005045.1 | + | 24981 | 0.66 | 0.686019 |
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Target: 5'- uUGAAGUGACgGcCCAgcGGGUGGGcUGc -3' miRNA: 3'- uGCUUCACUGgCuGGU--CCCACUC-ACc -5' |
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| 22315 | 3' | -55 | NC_005045.1 | + | 770 | 0.66 | 0.674993 |
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Target: 5'- gGCuGGGUGGCUGcucCCuGGGUG-GUGGa -3' miRNA: 3'- -UGcUUCACUGGCu--GGuCCCACuCACC- -5' |
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| 22315 | 3' | -55 | NC_005045.1 | + | 21885 | 0.67 | 0.630584 |
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Target: 5'- cCGAAGUGACUGcuGCCuuGGcucGGGUGGa -3' miRNA: 3'- uGCUUCACUGGC--UGGucCCa--CUCACC- -5' |
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| 22315 | 3' | -55 | NC_005045.1 | + | 12470 | 0.67 | 0.597252 |
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Target: 5'- gGCGAuccauCCG-CCAGGGUGAG-GGc -3' miRNA: 3'- -UGCUucacuGGCuGGUCCCACUCaCC- -5' |
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| 22315 | 3' | -55 | NC_005045.1 | + | 12151 | 1.09 | 0.00084 |
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Target: 5'- cACGAAGUGACCGACCAGGGUGAGUGGc -3' miRNA: 3'- -UGCUUCACUGGCUGGUCCCACUCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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