Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22316 | 3' | -58.3 | NC_005045.1 | + | 3859 | 0.66 | 0.524889 |
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Target: 5'- gGGGucAGGcguccUGCGGACAauaCCGGCGGg -3' miRNA: 3'- gCCC--UCCu----AUGCCUGUag-GGCCGCUg -5' |
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| 22316 | 3' | -58.3 | NC_005045.1 | + | 23682 | 0.66 | 0.483694 |
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Target: 5'- --cGAGGAUcGCGGu--UCUCGGCGGCu -3' miRNA: 3'- gccCUCCUA-UGCCuguAGGGCCGCUG- -5' |
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| 22316 | 3' | -58.3 | NC_005045.1 | + | 14119 | 0.66 | 0.483694 |
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Target: 5'- cCGGcGGuGGcgGCGGucGCAucuUCCCGGCGGg -3' miRNA: 3'- -GCC-CU-CCuaUGCC--UGU---AGGGCCGCUg -5' |
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| 22316 | 3' | -58.3 | NC_005045.1 | + | 12738 | 0.67 | 0.434508 |
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Target: 5'- aCGGGAGc-UGCuGGAgGUUCgGGCGGCu -3' miRNA: 3'- -GCCCUCcuAUG-CCUgUAGGgCCGCUG- -5' |
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| 22316 | 3' | -58.3 | NC_005045.1 | + | 25028 | 0.67 | 0.415658 |
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Target: 5'- uCGGGAGGAacaGGGCGaUCUGGCG-Cu -3' miRNA: 3'- -GCCCUCCUaugCCUGUaGGGCCGCuG- -5' |
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| 22316 | 3' | -58.3 | NC_005045.1 | + | 35410 | 0.68 | 0.406423 |
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Target: 5'- cCGGGGcGGAcaucCGGGagugCCCGGUGACu -3' miRNA: 3'- -GCCCU-CCUau--GCCUgua-GGGCCGCUG- -5' |
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| 22316 | 3' | -58.3 | NC_005045.1 | + | 29537 | 0.68 | 0.365647 |
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Target: 5'- -uGGAGG-UGCGcGACAagguaggcaugcaguUCCUGGCGGCc -3' miRNA: 3'- gcCCUCCuAUGC-CUGU---------------AGGGCCGCUG- -5' |
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| 22316 | 3' | -58.3 | NC_005045.1 | + | 18957 | 0.69 | 0.337375 |
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Target: 5'- cCGGGgcGGGGUGCuGGGCAUUCCGcaGCGcACc -3' miRNA: 3'- -GCCC--UCCUAUG-CCUGUAGGGC--CGC-UG- -5' |
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| 22316 | 3' | -58.3 | NC_005045.1 | + | 36964 | 0.69 | 0.329368 |
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Target: 5'- gCGGGgaccGGGGUACGGuuGCcgGUgCCGGCGAa -3' miRNA: 3'- -GCCC----UCCUAUGCC--UG--UAgGGCCGCUg -5' |
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| 22316 | 3' | -58.3 | NC_005045.1 | + | 12267 | 1.08 | 0.000453 |
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Target: 5'- cCGGGAGGAUACGGACAUCCCGGCGACc -3' miRNA: 3'- -GCCCUCCUAUGCCUGUAGGGCCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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