Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22316 | 5' | -55 | NC_005045.1 | + | 2800 | 0.66 | 0.650643 |
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Target: 5'- uUGgcGGCCAGCGcUUCCaGG-UCaCCCa -3' miRNA: 3'- -ACuaCUGGUCGCuAAGG-CCaAG-GGGc -5' |
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| 22316 | 5' | -55 | NC_005045.1 | + | 12770 | 0.67 | 0.5948 |
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Target: 5'- gGAUGAUCGGCGAgaCCGacuauaccgCCCUGg -3' miRNA: 3'- aCUACUGGUCGCUaaGGCcaa------GGGGC- -5' |
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| 22316 | 5' | -55 | NC_005045.1 | + | 23536 | 0.68 | 0.561606 |
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Target: 5'- gUGAaGGCCGGCGGcggcgggacUUCCaGGUUCUCgGg -3' miRNA: 3'- -ACUaCUGGUCGCU---------AAGG-CCAAGGGgC- -5' |
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| 22316 | 5' | -55 | NC_005045.1 | + | 25738 | 0.68 | 0.550647 |
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Target: 5'- cGAUGACCugGGCGGUcucgUCCGGUccaaUCUCg- -3' miRNA: 3'- aCUACUGG--UCGCUA----AGGCCA----AGGGgc -5' |
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| 22316 | 5' | -55 | NC_005045.1 | + | 7989 | 0.68 | 0.528937 |
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Target: 5'- gUGAUGcGCCGGC---UCCGG-UCCCUGc -3' miRNA: 3'- -ACUAC-UGGUCGcuaAGGCCaAGGGGC- -5' |
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| 22316 | 5' | -55 | NC_005045.1 | + | 6081 | 0.69 | 0.486538 |
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Target: 5'- gGA-GACuCAGCGAUUgcugaCGGUggCCCCGa -3' miRNA: 3'- aCUaCUG-GUCGCUAAg----GCCAa-GGGGC- -5' |
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| 22316 | 5' | -55 | NC_005045.1 | + | 7200 | 0.7 | 0.445819 |
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Target: 5'- cGAUGACCAGCGGUUU--GUUCUCg- -3' miRNA: 3'- aCUACUGGUCGCUAAGgcCAAGGGgc -5' |
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| 22316 | 5' | -55 | NC_005045.1 | + | 28755 | 0.74 | 0.267839 |
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Target: 5'- cUGGUGGCCGGUcuGAUgucggCgGGUUCCCUGu -3' miRNA: 3'- -ACUACUGGUCG--CUAa----GgCCAAGGGGC- -5' |
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| 22316 | 5' | -55 | NC_005045.1 | + | 12302 | 1.1 | 0.000692 |
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Target: 5'- aUGAUGACCAGCGAUUCCGGUUCCCCGg -3' miRNA: 3'- -ACUACUGGUCGCUAAGGCCAAGGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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