Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22317 | 3' | -59.4 | NC_005045.1 | + | 15745 | 0.66 | 0.434508 |
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Target: 5'- gUGUCGccgcCCAGCCacugcGCCaggaaGAACUUCCGa -3' miRNA: 3'- gACAGCa---GGUCGG-----CGGg----CUUGGAGGU- -5' |
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| 22317 | 3' | -59.4 | NC_005045.1 | + | 23465 | 0.67 | 0.379507 |
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Target: 5'- cCUGgaaGUCCcGCCGCCgCcGGCCUUCAc -3' miRNA: 3'- -GACag-CAGGuCGGCGG-GcUUGGAGGU- -5' |
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| 22317 | 3' | -59.4 | NC_005045.1 | + | 29380 | 0.69 | 0.263644 |
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Target: 5'- --aUCGaCCAgGCCGCCCGuGGCUUCCGu -3' miRNA: 3'- gacAGCaGGU-CGGCGGGC-UUGGAGGU- -5' |
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| 22317 | 3' | -59.4 | NC_005045.1 | + | 16943 | 0.7 | 0.250568 |
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Target: 5'- -gGUCGUCCAGCggggCGCCCuGGCagUCCAu -3' miRNA: 3'- gaCAGCAGGUCG----GCGGGcUUGg-AGGU- -5' |
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| 22317 | 3' | -59.4 | NC_005045.1 | + | 18122 | 0.7 | 0.244232 |
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Target: 5'- -cGUCGUUCugcGCCGCCCcgacaaGGGCCUCUu -3' miRNA: 3'- gaCAGCAGGu--CGGCGGG------CUUGGAGGu -5' |
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| 22317 | 3' | -59.4 | NC_005045.1 | + | 22247 | 0.71 | 0.208976 |
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Target: 5'- gUGUacagGUCCAcGCUGCCCGAACCggaCAg -3' miRNA: 3'- gACAg---CAGGU-CGGCGGGCUUGGag-GU- -5' |
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| 22317 | 3' | -59.4 | NC_005045.1 | + | 17818 | 0.73 | 0.151514 |
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Target: 5'- -cGUCGUCCAGCgGCCCG----UCCAa -3' miRNA: 3'- gaCAGCAGGUCGgCGGGCuuggAGGU- -5' |
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| 22317 | 3' | -59.4 | NC_005045.1 | + | 29503 | 0.73 | 0.151514 |
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Target: 5'- -cGUCG-CCGGUgGCCUGAGCCUgCAc -3' miRNA: 3'- gaCAGCaGGUCGgCGGGCUUGGAgGU- -5' |
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| 22317 | 3' | -59.4 | NC_005045.1 | + | 6121 | 0.75 | 0.114692 |
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Target: 5'- -cGUCGUCCcaauacaGGCCgGCCCGuugaccuACCUCCAc -3' miRNA: 3'- gaCAGCAGG-------UCGG-CGGGCu------UGGAGGU- -5' |
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| 22317 | 3' | -59.4 | NC_005045.1 | + | 12660 | 1.08 | 0.000347 |
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Target: 5'- uCUGUCGUCCAGCCGCCCGAACCUCCAg -3' miRNA: 3'- -GACAGCAGGUCGGCGGGCUUGGAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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