Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22319 | 5' | -55.8 | NC_005045.1 | + | 29905 | 0.66 | 0.553582 |
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Target: 5'- ---aUGGCGGCCCUGGaa-GCGGGGGa -3' miRNA: 3'- uaugACCGCUGGGACCcgaUGUUCUU- -5' |
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| 22319 | 5' | -55.8 | NC_005045.1 | + | 11806 | 0.66 | 0.531234 |
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Target: 5'- -gGCUGGCua-CCUGGagcGCUGCGAGGc -3' miRNA: 3'- uaUGACCGcugGGACC---CGAUGUUCUu -5' |
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| 22319 | 5' | -55.8 | NC_005045.1 | + | 20938 | 0.67 | 0.456014 |
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Target: 5'- uGUGCUGGCuGGCaguaCaGGGCUGgAAGAGu -3' miRNA: 3'- -UAUGACCG-CUGg---GaCCCGAUgUUCUU- -5' |
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| 22319 | 5' | -55.8 | NC_005045.1 | + | 35960 | 0.68 | 0.405947 |
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Target: 5'- -cGCUGcGCcguGCCCUGGGCUucgcCGAGAu -3' miRNA: 3'- uaUGAC-CGc--UGGGACCCGAu---GUUCUu -5' |
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| 22319 | 5' | -55.8 | NC_005045.1 | + | 41800 | 0.69 | 0.350643 |
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Target: 5'- -cGCUGgaagaaGCGGCCCUGGGUUcuggcuacaACGAGAc -3' miRNA: 3'- uaUGAC------CGCUGGGACCCGA---------UGUUCUu -5' |
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| 22319 | 5' | -55.8 | NC_005045.1 | + | 32138 | 0.71 | 0.270884 |
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Target: 5'- cUGCUGGCGGCgCUGGGCaaguCGGGu- -3' miRNA: 3'- uAUGACCGCUGgGACCCGau--GUUCuu -5' |
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| 22319 | 5' | -55.8 | NC_005045.1 | + | 7172 | 0.71 | 0.270884 |
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Target: 5'- --uCUGGUGGCCCUGGGCgUGCu---- -3' miRNA: 3'- uauGACCGCUGGGACCCG-AUGuucuu -5' |
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| 22319 | 5' | -55.8 | NC_005045.1 | + | 8792 | 0.72 | 0.249983 |
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Target: 5'- uGUACUcGGCGGCCCUGGGUaugACcguGGGu -3' miRNA: 3'- -UAUGA-CCGCUGGGACCCGa--UGu--UCUu -5' |
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| 22319 | 5' | -55.8 | NC_005045.1 | + | 16908 | 0.72 | 0.243318 |
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Target: 5'- cUGCUGGUGGCCCUGcacaaGCUGCuGGAc -3' miRNA: 3'- uAUGACCGCUGGGACc----CGAUGuUCUu -5' |
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| 22319 | 5' | -55.8 | NC_005045.1 | + | 13870 | 1.04 | 0.001072 |
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Target: 5'- cAUACUGGCGACCCUGGGCUACAAGAAc -3' miRNA: 3'- -UAUGACCGCUGGGACCCGAUGUUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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