Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22323 | 5' | -57.5 | NC_005045.1 | + | 28530 | 0.66 | 0.514944 |
Target: 5'- gGCGGAagcgcCGAGGcucaggcCGGGGCguUCGGCGUc -3' miRNA: 3'- -UGCCU-----GCUCCu------GCUCCGguAGCUGUA- -5' |
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22323 | 5' | -57.5 | NC_005045.1 | + | 41289 | 0.66 | 0.503388 |
Target: 5'- aGCGGGCGccGGGGCGguugcacAGGCUGUcaaCGACAg -3' miRNA: 3'- -UGCCUGC--UCCUGC-------UCCGGUA---GCUGUa -5' |
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22323 | 5' | -57.5 | NC_005045.1 | + | 28045 | 0.66 | 0.494017 |
Target: 5'- cACGGGuCGAGGucAUGggcAGGCCAUUGugGUc -3' miRNA: 3'- -UGCCU-GCUCC--UGC---UCCGGUAGCugUA- -5' |
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22323 | 5' | -57.5 | NC_005045.1 | + | 12701 | 0.66 | 0.4837 |
Target: 5'- -aGGACGAGGACGAcccGGaaGcCGGCAg -3' miRNA: 3'- ugCCUGCUCCUGCU---CCggUaGCUGUa -5' |
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22323 | 5' | -57.5 | NC_005045.1 | + | 15233 | 0.68 | 0.386976 |
Target: 5'- gUGGccgcagccACGuAGG-CGGGGCCGUCGGCGUc -3' miRNA: 3'- uGCC--------UGC-UCCuGCUCCGGUAGCUGUA- -5' |
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22323 | 5' | -57.5 | NC_005045.1 | + | 15823 | 0.69 | 0.343627 |
Target: 5'- -gGGugGAGGAUGucgaAGGCCGUCuuCAUg -3' miRNA: 3'- ugCCugCUCCUGC----UCCGGUAGcuGUA- -5' |
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22323 | 5' | -57.5 | NC_005045.1 | + | 35243 | 0.69 | 0.335387 |
Target: 5'- aGCGGauacuggaauGCGAGGAgCGAGGCCA-CGcCAa -3' miRNA: 3'- -UGCC----------UGCUCCU-GCUCCGGUaGCuGUa -5' |
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22323 | 5' | -57.5 | NC_005045.1 | + | 6203 | 0.69 | 0.327293 |
Target: 5'- aGCGGGCGAaGGCGGcGGCUAagGACAc -3' miRNA: 3'- -UGCCUGCUcCUGCU-CCGGUagCUGUa -5' |
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22323 | 5' | -57.5 | NC_005045.1 | + | 9272 | 0.7 | 0.319345 |
Target: 5'- gAUGGACGGGaagccuucgaacGugGAGGCCAugauuaacuUCGACGc -3' miRNA: 3'- -UGCCUGCUC------------CugCUCCGGU---------AGCUGUa -5' |
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22323 | 5' | -57.5 | NC_005045.1 | + | 27420 | 0.72 | 0.223137 |
Target: 5'- gUGGACGGcGACGGGGCCAUCu-CAUg -3' miRNA: 3'- uGCCUGCUcCUGCUCCGGUAGcuGUA- -5' |
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22323 | 5' | -57.5 | NC_005045.1 | + | 33948 | 0.73 | 0.206027 |
Target: 5'- aGCGGGCGcGGGACaGGGCUAcCGGCAUc -3' miRNA: 3'- -UGCCUGC-UCCUGcUCCGGUaGCUGUA- -5' |
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22323 | 5' | -57.5 | NC_005045.1 | + | 15786 | 1.06 | 0.000738 |
Target: 5'- gACGGACGAGGACGAGGCCAUCGACAUg -3' miRNA: 3'- -UGCCUGCUCCUGCUCCGGUAGCUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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