Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22326 | 3' | -59.4 | NC_005045.1 | + | 42298 | 0.66 | 0.392099 |
Target: 5'- -cGcAGGGCGCUGGGUAUgaG-CCGAu -3' miRNA: 3'- uuCuUCCCGCGGCUCGUGgaCuGGCU- -5' |
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22326 | 3' | -59.4 | NC_005045.1 | + | 235 | 0.66 | 0.392099 |
Target: 5'- -cGcAGGGCGCUGGGUAUgaG-CCGAu -3' miRNA: 3'- uuCuUCCCGCGGCUCGUGgaCuGGCU- -5' |
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22326 | 3' | -59.4 | NC_005045.1 | + | 9471 | 0.66 | 0.383086 |
Target: 5'- --cGAGGGCGUC--GC-CCUGGCCGGu -3' miRNA: 3'- uucUUCCCGCGGcuCGuGGACUGGCU- -5' |
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22326 | 3' | -59.4 | NC_005045.1 | + | 30963 | 0.66 | 0.383086 |
Target: 5'- -cGAugcGGGGCGCCGAGUACgguGCCa- -3' miRNA: 3'- uuCU---UCCCGCGGCUCGUGgacUGGcu -5' |
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22326 | 3' | -59.4 | NC_005045.1 | + | 40843 | 0.66 | 0.374212 |
Target: 5'- --uGAGGGCuGCCaAGC-CCUGGCCa- -3' miRNA: 3'- uucUUCCCG-CGGcUCGuGGACUGGcu -5' |
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22326 | 3' | -59.4 | NC_005045.1 | + | 24025 | 0.66 | 0.373332 |
Target: 5'- cAGGGcaGGUGCCGcAGCACCcagggguUGGCCGGc -3' miRNA: 3'- -UUCUucCCGCGGC-UCGUGG-------ACUGGCU- -5' |
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22326 | 3' | -59.4 | NC_005045.1 | + | 33437 | 0.67 | 0.356886 |
Target: 5'- cAGGA-GGCGCCGuGCGCCcucuccgGGCUGGg -3' miRNA: 3'- uUCUUcCCGCGGCuCGUGGa------CUGGCU- -5' |
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22326 | 3' | -59.4 | NC_005045.1 | + | 30242 | 0.67 | 0.348437 |
Target: 5'- cGAGcAGGGCGCCGuG-GCCUGGgCGc -3' miRNA: 3'- -UUCuUCCCGCGGCuCgUGGACUgGCu -5' |
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22326 | 3' | -59.4 | NC_005045.1 | + | 23280 | 0.67 | 0.323954 |
Target: 5'- cAGGuGGGuGCGCCGcAGCAaCUGGCCa- -3' miRNA: 3'- -UUCuUCC-CGCGGC-UCGUgGACUGGcu -5' |
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22326 | 3' | -59.4 | NC_005045.1 | + | 28963 | 0.67 | 0.323954 |
Target: 5'- ------cGCGCCGAGCACuCUGGCCc- -3' miRNA: 3'- uucuuccCGCGGCUCGUG-GACUGGcu -5' |
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22326 | 3' | -59.4 | NC_005045.1 | + | 35975 | 0.68 | 0.316083 |
Target: 5'- ----uGGGCuucGCCGAgauucGCACCgUGACCGAg -3' miRNA: 3'- uucuuCCCG---CGGCU-----CGUGG-ACUGGCU- -5' |
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22326 | 3' | -59.4 | NC_005045.1 | + | 22218 | 0.68 | 0.303791 |
Target: 5'- cAAGAAGGGCacggcgauggaguacGCCGAGCuguACCacgaGAUCGAc -3' miRNA: 3'- -UUCUUCCCG---------------CGGCUCG---UGGa---CUGGCU- -5' |
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22326 | 3' | -59.4 | NC_005045.1 | + | 33372 | 0.69 | 0.258307 |
Target: 5'- gGAG-AGGGCGCaCG-GCGCCUccugGGCCGu -3' miRNA: 3'- -UUCuUCCCGCG-GCuCGUGGA----CUGGCu -5' |
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22326 | 3' | -59.4 | NC_005045.1 | + | 16628 | 0.69 | 0.238975 |
Target: 5'- cAAGAAGGGCGCgcuGCACUggcugGGCCGc -3' miRNA: 3'- -UUCUUCCCGCGgcuCGUGGa----CUGGCu -5' |
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22326 | 3' | -59.4 | NC_005045.1 | + | 12465 | 0.71 | 0.176787 |
Target: 5'- gGAGAaacAGGGCuucgaggcggccaaGCUGuugauGCGCCUGGCCGAg -3' miRNA: 3'- -UUCU---UCCCG--------------CGGCu----CGUGGACUGGCU- -5' |
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22326 | 3' | -59.4 | NC_005045.1 | + | 16508 | 1.06 | 0.000382 |
Target: 5'- cAAGAAGGGCGCCGAGCACCUGACCGAg -3' miRNA: 3'- -UUCUUCCCGCGGCUCGUGGACUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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