Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22327 | 5' | -50 | NC_005045.1 | + | 33371 | 0.66 | 0.904359 |
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Target: 5'- -cGGAGag---GGCGCACGGCGCc-- -3' miRNA: 3'- gaCCUCauguaCUGCGUGUUGCGaac -5' |
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| 22327 | 5' | -50 | NC_005045.1 | + | 22192 | 0.66 | 0.904359 |
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Target: 5'- gUGGAccuGUACAcccACGUACAGCGCa-- -3' miRNA: 3'- gACCU---CAUGUac-UGCGUGUUGCGaac -5' |
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| 22327 | 5' | -50 | NC_005045.1 | + | 9708 | 0.66 | 0.887129 |
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Target: 5'- cCUGGAGgGCAUgGACGUggauuuuaacuacuACGACGCc-- -3' miRNA: 3'- -GACCUCaUGUA-CUGCG--------------UGUUGCGaac -5' |
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| 22327 | 5' | -50 | NC_005045.1 | + | 36547 | 0.68 | 0.818672 |
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Target: 5'- uCUGGAGUAUG-GGCGUGCugAGCGCg-- -3' miRNA: 3'- -GACCUCAUGUaCUGCGUG--UUGCGaac -5' |
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| 22327 | 5' | -50 | NC_005045.1 | + | 9632 | 0.69 | 0.777624 |
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Target: 5'- gCUGGAGUACcgaGuACGC-CGACGCa-- -3' miRNA: 3'- -GACCUCAUGua-C-UGCGuGUUGCGaac -5' |
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| 22327 | 5' | -50 | NC_005045.1 | + | 17092 | 1.08 | 0.002669 |
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Target: 5'- gCUGGAGUACAUGACGCACAACGCUUGc -3' miRNA: 3'- -GACCUCAUGUACUGCGUGUUGCGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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