Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22331 | 3' | -61.4 | NC_005045.1 | + | 39576 | 0.66 | 0.277383 |
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Target: 5'- ----cGGCGCUGauGGCUGGagGCUGg- -3' miRNA: 3'- caguaCCGCGACgcCCGACC--CGACag -5' |
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| 22331 | 3' | -61.4 | NC_005045.1 | + | 40012 | 0.67 | 0.257008 |
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Target: 5'- --gGUGGCGCUGCaugauGGGCcgcaGGG-UGUCg -3' miRNA: 3'- cagUACCGCGACG-----CCCGa---CCCgACAG- -5' |
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| 22331 | 3' | -61.4 | NC_005045.1 | + | 41983 | 0.68 | 0.219946 |
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Target: 5'- ----gGGUGUgUGUGGGCUGGGUUGg- -3' miRNA: 3'- caguaCCGCG-ACGCCCGACCCGACag -5' |
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| 22331 | 3' | -61.4 | NC_005045.1 | + | 41820 | 0.69 | 0.186038 |
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Target: 5'- gGUUcUGGCuacaacgagaccaaGCUGCGGGCUGaGCUgGUCg -3' miRNA: 3'- -CAGuACCG--------------CGACGCCCGACcCGA-CAG- -5' |
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| 22331 | 3' | -61.4 | NC_005045.1 | + | 23393 | 0.7 | 0.158116 |
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Target: 5'- cGUCAUGGCGCuggucggaacagguUGCGGcuGCUGGGUcGg- -3' miRNA: 3'- -CAGUACCGCG--------------ACGCC--CGACCCGaCag -5' |
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| 22331 | 3' | -61.4 | NC_005045.1 | + | 32129 | 0.71 | 0.127851 |
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Target: 5'- -gCAUGGCuGCUgcugGCGGcGCUGGGCaaGUCg -3' miRNA: 3'- caGUACCG-CGA----CGCC-CGACCCGa-CAG- -5' |
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| 22331 | 3' | -61.4 | NC_005045.1 | + | 20092 | 0.71 | 0.124338 |
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Target: 5'- ----gGGUccgGCUGCGGGCcGaGGCUGUCg -3' miRNA: 3'- caguaCCG---CGACGCCCGaC-CCGACAG- -5' |
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| 22331 | 3' | -61.4 | NC_005045.1 | + | 18268 | 1.09 | 0.000131 |
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Target: 5'- aGUCAUGGCGCUGCGGGCUGGGCUGUCg -3' miRNA: 3'- -CAGUACCGCGACGCCCGACCCGACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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