miRNA: 3'- -AGUU-GAUGGGUUgCUaGUGGcgCUGc -5'
miRNA: 3'- -AGUUG-AUGGGUUgCUAGUggCGCuGC- -5'
miRNA: 3'- -AGUUGAUg-GGUUGCU--AGuGGCGCUGC- -5'
miRNA: 3'- aguuGAUGGGUUGC-UAGU----------GGCG-CUGC- -5'
miRNA: 3'- aGUUGAUG------GGUUGCUaGUGGCgCUGc -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22331 | 5' | -51.7 | NC_005045.1 | + | 25454 | 0.66 | 0.828765 |
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Target: 5'- aUCAAcCUGCCCAAgGG-CACCuuGGCa -3' miRNA: 3'- -AGUU-GAUGGGUUgCUaGUGGcgCUGc -5' |
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| 22331 | 5' | -51.7 | NC_005045.1 | + | 16973 | 0.66 | 0.847012 |
|
Target: 5'- uUCGACgUGCCCAA-GAUCAauGCG-CGg -3' miRNA: 3'- -AGUUG-AUGGGUUgCUAGUggCGCuGC- -5' |
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| 22331 | 5' | -51.7 | NC_005045.1 | + | 28030 | 0.66 | 0.855778 |
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Target: 5'- uUCGGCUGgaCCGGCGAguUC-CUGUGGCGc -3' miRNA: 3'- -AGUUGAUg-GGUUGCU--AGuGGCGCUGC- -5' |
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| 22331 | 5' | -51.7 | NC_005045.1 | + | 8225 | 0.66 | 0.855778 |
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Target: 5'- ---uCUACCCAGCGcAUCAgaagguagacCCGCaGGCGc -3' miRNA: 3'- aguuGAUGGGUUGC-UAGU----------GGCG-CUGC- -5' |
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| 22331 | 5' | -51.7 | NC_005045.1 | + | 30725 | 0.66 | 0.864294 |
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Target: 5'- aCAGCUAUucgggaCCAACGA-CACCGaGACc -3' miRNA: 3'- aGUUGAUG------GGUUGCUaGUGGCgCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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