miRNA: 3'- -AGUUGAUG-GGUUgCUAGUGGCGcUGc -5'
miRNA: 3'- -AGUUGa----UG--GGUUgCuAGUGGCGCUGc -5'
miRNA: 3'- aGUUG--AUGGGUUGCuAGUGgCGCUgc -5'
miRNA: 3'- aGUUGAUGG-GUUgCUaGUGGCGcUGc -5'
miRNA: 3'- -AGUUGA-----UGGGUUgC-UAGUGGC---GCUGC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22331 | 5' | -51.7 | NC_005045.1 | + | 38933 | 0.68 | 0.758529 |
|
Target: 5'- aUCAACUGCaUCGG-GAUCACCGCcGCc -3' miRNA: 3'- -AGUUGAUG-GGUUgCUAGUGGCGcUGc -5' |
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| 22331 | 5' | -51.7 | NC_005045.1 | + | 2056 | 0.68 | 0.758529 |
|
Target: 5'- gUCAACgcaaaGCcgCCAAgGcUCGCCGCGACa -3' miRNA: 3'- -AGUUGa----UG--GGUUgCuAGUGGCGCUGc -5' |
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| 22331 | 5' | -51.7 | NC_005045.1 | + | 14832 | 0.68 | 0.747843 |
|
Target: 5'- cCGGCagUGCCCAACG-UCGCgGCGGa- -3' miRNA: 3'- aGUUG--AUGGGUUGCuAGUGgCGCUgc -5' |
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| 22331 | 5' | -51.7 | NC_005045.1 | + | 13495 | 0.68 | 0.747843 |
|
Target: 5'- cCGACUGCCaCAAgGA-CACCGCcGCc -3' miRNA: 3'- aGUUGAUGG-GUUgCUaGUGGCGcUGc -5' |
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| 22331 | 5' | -51.7 | NC_005045.1 | + | 26960 | 0.71 | 0.590696 |
|
Target: 5'- uUCAACUucgucACCCGAgGcAUCACCGgcaUGACGa -3' miRNA: 3'- -AGUUGA-----UGGGUUgC-UAGUGGC---GCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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