Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22333 | 3' | -61.1 | NC_005045.1 | + | 9686 | 0.66 | 0.404134 |
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Target: 5'- uGUCCcUCguaGGcuGCCGUgGCCuGGAGGg -3' miRNA: 3'- gCAGGuGGa--CCu-CGGCAgCGG-CCUCC- -5' |
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| 22333 | 3' | -61.1 | NC_005045.1 | + | 7002 | 0.66 | 0.38396 |
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Target: 5'- aGUU--CCUGGAGCCGUUcuacgccgacuuggGCUGGAGc -3' miRNA: 3'- gCAGguGGACCUCGGCAG--------------CGGCCUCc -5' |
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| 22333 | 3' | -61.1 | NC_005045.1 | + | 5344 | 0.66 | 0.361146 |
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Target: 5'- uCGgCgGCCUGGAGCCGUCGgCucaacAGGu -3' miRNA: 3'- -GCaGgUGGACCUCGGCAGCgGcc---UCC- -5' |
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| 22333 | 3' | -61.1 | NC_005045.1 | + | 39721 | 0.67 | 0.332225 |
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Target: 5'- gCGUgCCGCCcagGGcauAcgacagcucgucgccGCCGUCGCCGGcAGGg -3' miRNA: 3'- -GCA-GGUGGa--CC---U---------------CGGCAGCGGCC-UCC- -5' |
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| 22333 | 3' | -61.1 | NC_005045.1 | + | 15407 | 0.69 | 0.258423 |
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Target: 5'- aCGUgUACgaGcGAGgCGgggCGCCGGAGGg -3' miRNA: 3'- -GCAgGUGgaC-CUCgGCa--GCGGCCUCC- -5' |
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| 22333 | 3' | -61.1 | NC_005045.1 | + | 11696 | 0.69 | 0.25208 |
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Target: 5'- cCGUCCACCUugugGGuGCCGcCGUacuuCGGAGc -3' miRNA: 3'- -GCAGGUGGA----CCuCGGCaGCG----GCCUCc -5' |
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| 22333 | 3' | -61.1 | NC_005045.1 | + | 4824 | 0.7 | 0.211215 |
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Target: 5'- cCGagCGCCUGuccAGCCucGUCGCCGGAGa -3' miRNA: 3'- -GCagGUGGACc--UCGG--CAGCGGCCUCc -5' |
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| 22333 | 3' | -61.1 | NC_005045.1 | + | 22420 | 0.7 | 0.205866 |
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Target: 5'- -cUCgGCCUGuacGAGCUggaGUCGCUGGAGGu -3' miRNA: 3'- gcAGgUGGAC---CUCGG---CAGCGGCCUCC- -5' |
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| 22333 | 3' | -61.1 | NC_005045.1 | + | 18624 | 1.09 | 0.000251 |
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Target: 5'- gCGUCCACCUGGAGCCGUCGCCGGAGGc -3' miRNA: 3'- -GCAGGUGGACCUCGGCAGCGGCCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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