Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22334 | 5' | -55.5 | NC_005045.1 | + | 8580 | 0.66 | 0.592673 |
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Target: 5'- cAAGUugCcgGgGCUGGUGCuGCCgGCGu -3' miRNA: 3'- cUUCGugGuaCaCGACCAUG-CGG-CGU- -5' |
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| 22334 | 5' | -55.5 | NC_005045.1 | + | 23271 | 0.66 | 0.581379 |
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Target: 5'- -cAGC-CCGgucagGUgGGUGCGCCGCAg -3' miRNA: 3'- cuUCGuGGUaca--CGaCCAUGCGGCGU- -5' |
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| 22334 | 5' | -55.5 | NC_005045.1 | + | 10577 | 0.66 | 0.581379 |
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Target: 5'- cGAAGC-CCAUGgcgcGgUGGUA-GCCGUAc -3' miRNA: 3'- -CUUCGuGGUACa---CgACCAUgCGGCGU- -5' |
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| 22334 | 5' | -55.5 | NC_005045.1 | + | 4564 | 0.66 | 0.57013 |
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Target: 5'- cGAGUgACCAaGgcgaagGCUGGUGCGCCaGCc -3' miRNA: 3'- cUUCG-UGGUaCa-----CGACCAUGCGG-CGu -5' |
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| 22334 | 5' | -55.5 | NC_005045.1 | + | 34516 | 0.66 | 0.57013 |
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Target: 5'- -cAGCAUgGUGgcGCUGGUcAgGCCGCu -3' miRNA: 3'- cuUCGUGgUACa-CGACCA-UgCGGCGu -5' |
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| 22334 | 5' | -55.5 | NC_005045.1 | + | 32145 | 0.68 | 0.481699 |
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Target: 5'- -cGGCGCUgggcaagucggguAUGuUGCUGGgcuggAUGCCGCAg -3' miRNA: 3'- cuUCGUGG-------------UAC-ACGACCa----UGCGGCGU- -5' |
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| 22334 | 5' | -55.5 | NC_005045.1 | + | 8628 | 0.68 | 0.441517 |
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Target: 5'- cGggGC-CUcgGUGCUGGUggcaGCGCCu-- -3' miRNA: 3'- -CuuCGuGGuaCACGACCA----UGCGGcgu -5' |
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| 22334 | 5' | -55.5 | NC_005045.1 | + | 18777 | 1.08 | 0.000627 |
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Target: 5'- aGAAGCACCAUGUGCUGGUACGCCGCAc -3' miRNA: 3'- -CUUCGUGGUACACGACCAUGCGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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