Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22337 | 3' | -58.7 | NC_005045.1 | + | 36399 | 0.71 | 0.228425 |
Target: 5'- gCACcGGCuuggGCCGGCgaaUCuGCGACGCCa -3' miRNA: 3'- -GUGaCCG----UGGCCGug-AGcUGCUGCGG- -5' |
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22337 | 3' | -58.7 | NC_005045.1 | + | 10695 | 0.72 | 0.211246 |
Target: 5'- aGCUGGaagaugguuCCGGCAC-CGugGcCGCCa -3' miRNA: 3'- gUGACCgu-------GGCCGUGaGCugCuGCGG- -5' |
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22337 | 3' | -58.7 | NC_005045.1 | + | 8465 | 0.73 | 0.170803 |
Target: 5'- aGCU-GCGCCGGCugagCGACGAgGCCc -3' miRNA: 3'- gUGAcCGUGGCCGuga-GCUGCUgCGG- -5' |
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22337 | 3' | -58.7 | NC_005045.1 | + | 7030 | 0.74 | 0.15751 |
Target: 5'- aUACUcGGCACCaGCcaGCUCGGCGGC-CCg -3' miRNA: 3'- -GUGA-CCGUGGcCG--UGAGCUGCUGcGG- -5' |
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22337 | 3' | -58.7 | NC_005045.1 | + | 11590 | 0.77 | 0.093085 |
Target: 5'- gACgUGGCuguacCCGGCACUCGACGA-GCUg -3' miRNA: 3'- gUG-ACCGu----GGCCGUGAGCUGCUgCGG- -5' |
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22337 | 3' | -58.7 | NC_005045.1 | + | 21514 | 1.12 | 0.000234 |
Target: 5'- cCACUGGCACCGGCACUCGACGACGCCg -3' miRNA: 3'- -GUGACCGUGGCCGUGAGCUGCUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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