Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2234 | 5' | -52.8 | NC_001405.1 | + | 34686 | 0.66 | 0.700469 |
Target: 5'- -aAGGUgGAGG---GCGCUCUGgaGCa -3' miRNA: 3'- agUCCAaUUCCgagUGCGAGACgaCG- -5' |
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2234 | 5' | -52.8 | NC_001405.1 | + | 25092 | 0.67 | 0.670458 |
Target: 5'- gUCGGc---AGGCUCGCGCgUCUcaagccagcgcgccaGCUGCu -3' miRNA: 3'- -AGUCcaauUCCGAGUGCG-AGA---------------CGACG- -5' |
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2234 | 5' | -52.8 | NC_001405.1 | + | 12742 | 0.67 | 0.642506 |
Target: 5'- cCAGGUUGcccuGCUgcugcgCGCGCUCacGCUGCg -3' miRNA: 3'- aGUCCAAUuc--CGA------GUGCGAGa-CGACG- -5' |
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2234 | 5' | -52.8 | NC_001405.1 | + | 33188 | 0.67 | 0.627329 |
Target: 5'- aUCAGGaUAGGGCgguggugcugcagcaGCGCgcgaauaaaCUGCUGCc -3' miRNA: 3'- -AGUCCaAUUCCGag-------------UGCGa--------GACGACG- -5' |
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2234 | 5' | -52.8 | NC_001405.1 | + | 26919 | 0.67 | 0.617991 |
Target: 5'- aCGGGUUcguuGGGCgccagacgcaGCGCUCcuccuccUGCUGCu -3' miRNA: 3'- aGUCCAAu---UCCGag--------UGCGAG-------ACGACG- -5' |
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2234 | 5' | -52.8 | NC_001405.1 | + | 5217 | 0.68 | 0.549762 |
Target: 5'- gCGGGUUGGGGCg---GCUUUcGCUGUa -3' miRNA: 3'- aGUCCAAUUCCGagugCGAGA-CGACG- -5' |
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2234 | 5' | -52.8 | NC_001405.1 | + | 10080 | 0.69 | 0.537265 |
Target: 5'- cCAGGUc--GGCgacaaCGCGCUCggcuaauauggccUGCUGCa -3' miRNA: 3'- aGUCCAauuCCGa----GUGCGAG-------------ACGACG- -5' |
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2234 | 5' | -52.8 | NC_001405.1 | + | 5321 | 0.71 | 0.420297 |
Target: 5'- gUCAcGGUgAAGGggUGCGCUCcggGCUGCg -3' miRNA: 3'- -AGU-CCAaUUCCgaGUGCGAGa--CGACG- -5' |
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2234 | 5' | -52.8 | NC_001405.1 | + | 2246 | 0.71 | 0.420297 |
Target: 5'- cUCGGGUUccauGGGCUC-UGCUcCUGCcGCc -3' miRNA: 3'- -AGUCCAAu---UCCGAGuGCGA-GACGaCG- -5' |
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2234 | 5' | -52.8 | NC_001405.1 | + | 12400 | 0.72 | 0.362816 |
Target: 5'- -uGGaGUUAAGGCcggacggCugGCUCUGCaGCg -3' miRNA: 3'- agUC-CAAUUCCGa------GugCGAGACGaCG- -5' |
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2234 | 5' | -52.8 | NC_001405.1 | + | 13330 | 1.14 | 0.000405 |
Target: 5'- aUCAGGUUAAGGCUCACGCUCUGCUGCa -3' miRNA: 3'- -AGUCCAAUUCCGAGUGCGAGACGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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