Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22340 | 5' | -55.7 | NC_005045.1 | + | 8629 | 0.66 | 0.608417 |
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Target: 5'- gGGGCCUcggUGCUGGuGGcaGCGCcUCCuGAUg -3' miRNA: 3'- -UCUGGA---ACGACCuUC--CGCGaAGG-CUG- -5' |
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| 22340 | 5' | -55.7 | NC_005045.1 | + | 18050 | 0.66 | 0.608417 |
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Target: 5'- gAGGcCCUUGUcgGGgcGGCGCagaaCGACg -3' miRNA: 3'- -UCU-GGAACGa-CCuuCCGCGaag-GCUG- -5' |
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| 22340 | 5' | -55.7 | NC_005045.1 | + | 8567 | 0.66 | 0.585898 |
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Target: 5'- gGGACCacggaaucaagUUGCcGGGgcuGGUGCUgCCGGCg -3' miRNA: 3'- -UCUGG-----------AACGaCCUu--CCGCGAaGGCUG- -5' |
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| 22340 | 5' | -55.7 | NC_005045.1 | + | 13268 | 0.66 | 0.585897 |
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Target: 5'- cGGCCccaucucGCU-GAAGGCGCcugCCGGCa -3' miRNA: 3'- uCUGGaa-----CGAcCUUCCGCGaa-GGCUG- -5' |
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| 22340 | 5' | -55.7 | NC_005045.1 | + | 2198 | 0.67 | 0.541417 |
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Target: 5'- -aGCCUUGC-GGAAgaucgggcagucGGCGCcUUUCGGCa -3' miRNA: 3'- ucUGGAACGaCCUU------------CCGCG-AAGGCUG- -5' |
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| 22340 | 5' | -55.7 | NC_005045.1 | + | 4888 | 0.67 | 0.51959 |
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Target: 5'- cGACgagGCUGGAcAGGCGCU--CGGCg -3' miRNA: 3'- uCUGgaaCGACCU-UCCGCGAagGCUG- -5' |
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| 22340 | 5' | -55.7 | NC_005045.1 | + | 15856 | 0.67 | 0.498119 |
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Target: 5'- gAGGCCgaggUGCUGuGGAuGCGCcgaacgCCGACu -3' miRNA: 3'- -UCUGGa---ACGAC-CUUcCGCGaa----GGCUG- -5' |
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| 22340 | 5' | -55.7 | NC_005045.1 | + | 28949 | 0.69 | 0.397649 |
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Target: 5'- uGGCCcgGCUGGAGuuGCGUcugUCCGACg -3' miRNA: 3'- uCUGGaaCGACCUUc-CGCGa--AGGCUG- -5' |
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| 22340 | 5' | -55.7 | NC_005045.1 | + | 7689 | 0.72 | 0.252827 |
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Target: 5'- cGACgCggGCUGGAuuGGGCGCgcuacugCCGACc -3' miRNA: 3'- uCUG-GaaCGACCU--UCCGCGaa-----GGCUG- -5' |
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| 22340 | 5' | -55.7 | NC_005045.1 | + | 23079 | 1.1 | 0.000444 |
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Target: 5'- gAGACCUUGCUGGAAGGCGCUUCCGACa -3' miRNA: 3'- -UCUGGAACGACCUUCCGCGAAGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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