Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22343 | 3' | -62.8 | NC_005045.1 | + | 32630 | 0.66 | 0.264967 |
Target: 5'- gAugCCCUGGaGcgGCUuaGGCACCUcgGCg -3' miRNA: 3'- -UugGGGACC-Ca-CGAcgCCGUGGA--CGg -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 2748 | 0.66 | 0.264967 |
Target: 5'- uGCCCUUGGu--CUGCGccuggaGCACCUGCUu -3' miRNA: 3'- uUGGGGACCcacGACGC------CGUGGACGG- -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 7179 | 0.66 | 0.262971 |
Target: 5'- --gCCCUGGGcgUGCUGCucacacagaacaacGGCGgCCcGCCu -3' miRNA: 3'- uugGGGACCC--ACGACG--------------CCGU-GGaCGG- -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 41519 | 0.66 | 0.245553 |
Target: 5'- --gCCCUGGGcgGCgaggGCGGCGCUaccCCa -3' miRNA: 3'- uugGGGACCCa-CGa---CGCCGUGGac-GG- -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 42122 | 0.66 | 0.243677 |
Target: 5'- uAGCCuCCUGGGaGCUuccggcuucgccggGUGGCGagggcuucCCUGCCc -3' miRNA: 3'- -UUGG-GGACCCaCGA--------------CGCCGU--------GGACGG- -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 59 | 0.66 | 0.243677 |
Target: 5'- uAGCCuCCUGGGaGCUuccggcuucgccggGUGGCGagggcuucCCUGCCc -3' miRNA: 3'- -UUGG-GGACCCaCGA--------------CGCCGU--------GGACGG- -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 15843 | 0.66 | 0.239348 |
Target: 5'- aAACCCgCUGgcggaggccgaGGUGCUGUGgauGCGCCgaacGCCg -3' miRNA: 3'- -UUGGG-GAC-----------CCACGACGC---CGUGGa---CGG- -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 26714 | 0.66 | 0.238735 |
Target: 5'- uGCCCCUgguguggGGGUGCaguucaugGaCGGCGCUgucaugGCCa -3' miRNA: 3'- uUGGGGA-------CCCACGa-------C-GCCGUGGa-----CGG- -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 362 | 0.67 | 0.227333 |
Target: 5'- uGCCuCCUGuGGUGguCUGUGGU-CCUGUCg -3' miRNA: 3'- uUGG-GGAC-CCAC--GACGCCGuGGACGG- -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 42425 | 0.67 | 0.227333 |
Target: 5'- uGCCuCCUGuGGUGguCUGUGGU-CCUGUCg -3' miRNA: 3'- uUGG-GGAC-CCAC--GACGCCGuGGACGG- -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 27268 | 0.67 | 0.225575 |
Target: 5'- cGACCUCgauaccaggGCGGCACCUGCCg -3' miRNA: 3'- -UUGGGGacccacga-CGCCGUGGACGG- -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 8783 | 0.67 | 0.223829 |
Target: 5'- gGGCCggCCUGGGUcgaaguaccgcuugaGCUGCGGCGCaaugUGUa -3' miRNA: 3'- -UUGG--GGACCCA---------------CGACGCCGUGg---ACGg -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 37641 | 0.67 | 0.204833 |
Target: 5'- uGCCCUUccaGGGUGC-GCGGgACCgGCg -3' miRNA: 3'- uUGGGGA---CCCACGaCGCCgUGGaCGg -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 13268 | 0.68 | 0.179444 |
Target: 5'- cGGCCCCaucucGCUGaaGGCGCCUGCCg -3' miRNA: 3'- -UUGGGGacccaCGACg-CCGUGGACGG- -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 32647 | 0.69 | 0.152694 |
Target: 5'- -uUCCgUGuGGUGCaggGCGGCGacaCUGCCa -3' miRNA: 3'- uuGGGgAC-CCACGa--CGCCGUg--GACGG- -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 23366 | 0.7 | 0.135803 |
Target: 5'- cGGCUgCUGGGUcgggauggccaguuGCUGCGGCgcacccACCUGaCCg -3' miRNA: 3'- -UUGGgGACCCA--------------CGACGCCG------UGGAC-GG- -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 3213 | 0.71 | 0.112885 |
Target: 5'- cGACCgcuaCCUGGGUGCUGaugaggcaGGCACCcGUg -3' miRNA: 3'- -UUGG----GGACCCACGACg-------CCGUGGaCGg -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 28141 | 0.72 | 0.098194 |
Target: 5'- gAGCCUCUGGuGgauagcGCUGUGGUGCCgcUGCCg -3' miRNA: 3'- -UUGGGGACC-Ca-----CGACGCCGUGG--ACGG- -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 587 | 0.73 | 0.071994 |
Target: 5'- cGCuCCCUGGGUGCUGgucuaccucccUGGCGCugugcuugugCUGCCg -3' miRNA: 3'- uUG-GGGACCCACGAC-----------GCCGUG----------GACGG- -5' |
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22343 | 3' | -62.8 | NC_005045.1 | + | 23954 | 1.07 | 0.000135 |
Target: 5'- cAACCCCUGGGUGCUGCGGCACCUGCCc -3' miRNA: 3'- -UUGGGGACCCACGACGCCGUGGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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