Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22347 | 5' | -50.7 | NC_005045.1 | + | 22028 | 0.66 | 0.877226 |
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Target: 5'- --aUCAGuAGCUUGAUcACCUGCGuCAGg -3' miRNA: 3'- agaAGUU-UCGGAUUAcUGGGCGC-GUC- -5' |
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| 22347 | 5' | -50.7 | NC_005045.1 | + | 35410 | 0.66 | 0.868909 |
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Target: 5'- --gUCAAGGaCCUAGUaaccggcgacGACCC-CGCAGc -3' miRNA: 3'- agaAGUUUC-GGAUUA----------CUGGGcGCGUC- -5' |
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| 22347 | 5' | -50.7 | NC_005045.1 | + | 32944 | 0.66 | 0.851453 |
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Target: 5'- uUCUcCAGGGUCUGcAUGGCCUGgGCc- -3' miRNA: 3'- -AGAaGUUUCGGAU-UACUGGGCgCGuc -5' |
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| 22347 | 5' | -50.7 | NC_005045.1 | + | 32378 | 0.66 | 0.842332 |
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Target: 5'- gCUUCAAGGCC-GGUGACgUG-GUAGa -3' miRNA: 3'- aGAAGUUUCGGaUUACUGgGCgCGUC- -5' |
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| 22347 | 5' | -50.7 | NC_005045.1 | + | 7165 | 0.67 | 0.832962 |
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Target: 5'- cCUUC--AGUCUGGUGGCCCuGgGCGu -3' miRNA: 3'- aGAAGuuUCGGAUUACUGGG-CgCGUc -5' |
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| 22347 | 5' | -50.7 | NC_005045.1 | + | 30376 | 0.68 | 0.750458 |
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Target: 5'- aCUUCGGcguuGGCgCUGA--ACUCGCGCAGg -3' miRNA: 3'- aGAAGUU----UCG-GAUUacUGGGCGCGUC- -5' |
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| 22347 | 5' | -50.7 | NC_005045.1 | + | 10471 | 0.69 | 0.716916 |
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Target: 5'- aUCUUCcauGAGgCUGcgGACCaugGCGCGGc -3' miRNA: 3'- -AGAAGu--UUCgGAUuaCUGGg--CGCGUC- -5' |
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| 22347 | 5' | -50.7 | NC_005045.1 | + | 23125 | 0.69 | 0.716916 |
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Target: 5'- gUCUccugCGcGGCCUG--GGCCUGCGCGGc -3' miRNA: 3'- -AGAa---GUuUCGGAUuaCUGGGCGCGUC- -5' |
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| 22347 | 5' | -50.7 | NC_005045.1 | + | 34246 | 0.72 | 0.532031 |
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Target: 5'- --gUC-GGGCCgcucagcauUGGCCCGCGCAGg -3' miRNA: 3'- agaAGuUUCGGauu------ACUGGGCGCGUC- -5' |
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| 22347 | 5' | -50.7 | NC_005045.1 | + | 25194 | 1.11 | 0.001505 |
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Target: 5'- uUCUUCAAAGCCUAAUGACCCGCGCAGa -3' miRNA: 3'- -AGAAGUUUCGGAUUACUGGGCGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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