Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22348 | 3' | -49.1 | NC_005045.1 | + | 25274 | 1.11 | 0.00194 |
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Target: 5'- gCAGUGAAUGUCAUCCUGCGCAAGAUCg -3' miRNA: 3'- -GUCACUUACAGUAGGACGCGUUCUAG- -5' |
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| 22348 | 3' | -49.1 | NC_005045.1 | + | 16169 | 0.68 | 0.836917 |
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Target: 5'- uGGUGcggGGUGUCcugcaccgGUCCUGCaacacugccgaagGCAAGAUCa -3' miRNA: 3'- gUCAC---UUACAG--------UAGGACG-------------CGUUCUAG- -5' |
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| 22348 | 3' | -49.1 | NC_005045.1 | + | 24941 | 0.68 | 0.837871 |
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Target: 5'- -cGUGAGUGcCAggagUCCgaGCGCcAGAUCg -3' miRNA: 3'- guCACUUACaGU----AGGa-CGCGuUCUAG- -5' |
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| 22348 | 3' | -49.1 | NC_005045.1 | + | 3819 | 0.66 | 0.905141 |
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Target: 5'- aGGUGAacucuaccacGUGaUCGUaCCUGC-CGAGGUCg -3' miRNA: 3'- gUCACU----------UAC-AGUA-GGACGcGUUCUAG- -5' |
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| 22348 | 3' | -49.1 | NC_005045.1 | + | 134 | 0.66 | 0.918928 |
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Target: 5'- gCGG-GAGUGUCAacCCUGCGguGGcgUg -3' miRNA: 3'- -GUCaCUUACAGUa-GGACGCguUCuaG- -5' |
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| 22348 | 3' | -49.1 | NC_005045.1 | + | 42197 | 0.66 | 0.918928 |
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Target: 5'- gCGG-GAGUGUCAacCCUGCGguGGcgUg -3' miRNA: 3'- -GUCaCUUACAGUa-GGACGCguUCuaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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