Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22350 | 3' | -56.7 | NC_005045.1 | + | 39015 | 0.66 | 0.535888 |
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Target: 5'- -cGGUagCCCUGGagGGCGGCg--GUGAUc -3' miRNA: 3'- caCCA--GGGGCCa-CCGCCGuaaCAUUG- -5' |
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| 22350 | 3' | -56.7 | NC_005045.1 | + | 6481 | 0.67 | 0.503729 |
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Target: 5'- cUGGgaCaaGGUGGCGGCGUgugccgGUAGCc -3' miRNA: 3'- cACCagGggCCACCGCCGUAa-----CAUUG- -5' |
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| 22350 | 3' | -56.7 | NC_005045.1 | + | 379 | 0.67 | 0.482761 |
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Target: 5'- uGUGGUCCUgucgGGUGGUGGUgcgggaGUGGCu -3' miRNA: 3'- -CACCAGGGg---CCACCGCCGuaa---CAUUG- -5' |
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| 22350 | 3' | -56.7 | NC_005045.1 | + | 42442 | 0.67 | 0.482761 |
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Target: 5'- uGUGGUCCUgucgGGUGGUGGUgcgggaGUGGCu -3' miRNA: 3'- -CACCAGGGg---CCACCGCCGuaa---CAUUG- -5' |
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| 22350 | 3' | -56.7 | NC_005045.1 | + | 40389 | 0.67 | 0.452128 |
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Target: 5'- -gGGUgccuuugcccgCCUCGGUGGCGGUAUcg-AGCa -3' miRNA: 3'- caCCA-----------GGGGCCACCGCCGUAacaUUG- -5' |
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| 22350 | 3' | -56.7 | NC_005045.1 | + | 28746 | 0.68 | 0.413001 |
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Target: 5'- -cGGUCCCCGcUGGUGGCcg-GUcuGAUg -3' miRNA: 3'- caCCAGGGGCcACCGCCGuaaCA--UUG- -5' |
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| 22350 | 3' | -56.7 | NC_005045.1 | + | 24498 | 0.68 | 0.403549 |
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Target: 5'- gGUGGUCgaaCUGGUGGCGGag--GUAcaGCa -3' miRNA: 3'- -CACCAGg--GGCCACCGCCguaaCAU--UG- -5' |
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| 22350 | 3' | -56.7 | NC_005045.1 | + | 4725 | 0.69 | 0.388716 |
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Target: 5'- gGUGGUUUCggUGGUGGUGGUguugacguuggucauGUUGUAGCu -3' miRNA: 3'- -CACCAGGG--GCCACCGCCG---------------UAACAUUG- -5' |
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| 22350 | 3' | -56.7 | NC_005045.1 | + | 15001 | 0.77 | 0.103636 |
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Target: 5'- gGUGG-CCCCGGUGGCGGCu------- -3' miRNA: 3'- -CACCaGGGGCCACCGCCGuaacauug -5' |
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| 22350 | 3' | -56.7 | NC_005045.1 | + | 27205 | 1.09 | 0.000443 |
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Target: 5'- cGUGGUCCCCGGUGGCGGCAUUGUAACu -3' miRNA: 3'- -CACCAGGGGCCACCGCCGUAACAUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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