miRNA: 3'- -GGCUUCugCAauGGUCGGu-----GUAG----GGC- -5'
miRNA: 3'- -GGCUUC----UGCaauGGUCGGUGUagGGC- -5'
miRNA: 3'- gGCUUCUGCaaUGGUcGgUGU---AGGGC- -5'
miRNA: 3'- -GGCuuCU--GCaa---UGGUCGGU-GUAGGGc -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22351 | 3' | -52.7 | NC_005045.1 | + | 39652 | 0.66 | 0.802268 |
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Target: 5'- gCUGGAGugGUgcCCAGCCuccagcCAUCagcgCCGg -3' miRNA: 3'- -GGCUUCugCAauGGUCGGu-----GUAG----GGC- -5' |
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| 22351 | 3' | -52.7 | NC_005045.1 | + | 20079 | 0.66 | 0.830835 |
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Target: 5'- aCCGAGGaaguGCGgguCCGGCUGCGggCCGa -3' miRNA: 3'- -GGCUUC----UGCaauGGUCGGUGUagGGC- -5' |
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| 22351 | 3' | -52.7 | NC_005045.1 | + | 15791 | 0.66 | 0.830835 |
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Target: 5'- aCGAGGACGagGCCAuCgACAugaUCCUGg -3' miRNA: 3'- gGCUUCUGCaaUGGUcGgUGU---AGGGC- -5' |
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| 22351 | 3' | -52.7 | NC_005045.1 | + | 12372 | 0.66 | 0.830835 |
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Target: 5'- gCCGggGAacCGgaaucGCUGGUCAuCAUCCCa -3' miRNA: 3'- -GGCuuCU--GCaa---UGGUCGGU-GUAGGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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