miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22354 5' -51.4 NC_005045.1 + 28649 1.09 0.001646
Target:  5'- gGACAAUCAGAUGUGGAACCUCGCCGAg -3'
miRNA:   3'- -CUGUUAGUCUACACCUUGGAGCGGCU- -5'
22354 5' -51.4 NC_005045.1 + 28680 0.67 0.787281
Target:  5'- -uCAAUCuGGAUcaGG-GCCUCGCCGAc -3'
miRNA:   3'- cuGUUAG-UCUAcaCCuUGGAGCGGCU- -5'
22354 5' -51.4 NC_005045.1 + 4508 0.67 0.797528
Target:  5'- aACGA-CGGGUGgcgcaUGGu-CCUCGCCGAc -3'
miRNA:   3'- cUGUUaGUCUAC-----ACCuuGGAGCGGCU- -5'
22354 5' -51.4 NC_005045.1 + 11171 0.67 0.797528
Target:  5'- cGACAGgugcaGGAUG-GGGACCUCGUg-- -3'
miRNA:   3'- -CUGUUag---UCUACaCCUUGGAGCGgcu -5'
22354 5' -51.4 NC_005045.1 + 39387 0.66 0.81744
Target:  5'- aGCu-UCAGuacAUGcUGGAcaccaGCCUCGCCGAc -3'
miRNA:   3'- cUGuuAGUC---UAC-ACCU-----UGGAGCGGCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.