Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22357 | 5' | -55.8 | NC_005045.1 | + | 11029 | 0.66 | 0.649317 |
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Target: 5'- gGGGGGAUaguAUCuGCGCCCUGg----- -3' miRNA: 3'- gCCCCCUG---UAGuUGCGGGAUgugugc -5' |
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| 22357 | 5' | -55.8 | NC_005045.1 | + | 32907 | 0.66 | 0.604964 |
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Target: 5'- cCGGGGuaACGugcUCAACGCCCUGaagaagacCGCGCc -3' miRNA: 3'- -GCCCCc-UGU---AGUUGCGGGAU--------GUGUGc -5' |
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| 22357 | 5' | -55.8 | NC_005045.1 | + | 34566 | 0.67 | 0.565349 |
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Target: 5'- cCGGuGGGcCAUCGugGCCagcucagcaagcgaUACACGCc -3' miRNA: 3'- -GCC-CCCuGUAGUugCGGg-------------AUGUGUGc -5' |
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| 22357 | 5' | -55.8 | NC_005045.1 | + | 11365 | 0.72 | 0.313247 |
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Target: 5'- gCGGGGGuCAaagggCGACGCCUUGCGCc-- -3' miRNA: 3'- -GCCCCCuGUa----GUUGCGGGAUGUGugc -5' |
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| 22357 | 5' | -55.8 | NC_005045.1 | + | 15430 | 0.75 | 0.190922 |
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Target: 5'- cCGGaGGGA-AUCGACGUuauCCUGCACACGu -3' miRNA: 3'- -GCC-CCCUgUAGUUGCG---GGAUGUGUGC- -5' |
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| 22357 | 5' | -55.8 | NC_005045.1 | + | 29920 | 0.98 | 0.004318 |
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Target: 5'- gCGGGGGACAUCAACGCCCUaACACGCu -3' miRNA: 3'- -GCCCCCUGUAGUUGCGGGA-UGUGUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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