Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22359 | 5' | -53.7 | NC_005045.1 | + | 40673 | 0.66 | 0.77972 |
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Target: 5'- gCGGUAGU-GCCggagcagauggUGGGUACG-CCGg -3' miRNA: 3'- aGCCAUCAgCGGaa---------GCUCAUGCuGGC- -5' |
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| 22359 | 5' | -53.7 | NC_005045.1 | + | 31679 | 0.66 | 0.761255 |
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Target: 5'- aCGGUAGgcacCGCg-UCGAGUugGcCCu -3' miRNA: 3'- aGCCAUCa---GCGgaAGCUCAugCuGGc -5' |
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| 22359 | 5' | -53.7 | NC_005045.1 | + | 40625 | 0.67 | 0.685885 |
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Target: 5'- gCGGUAGUCucacuGCCUUCcAGgGCGACa- -3' miRNA: 3'- aGCCAUCAG-----CGGAAGcUCaUGCUGgc -5' |
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| 22359 | 5' | -53.7 | NC_005045.1 | + | 7155 | 0.67 | 0.674811 |
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Target: 5'- aUCGG-AGUCGCCUUC-AGUcuggUGGCCc -3' miRNA: 3'- -AGCCaUCAGCGGAAGcUCAu---GCUGGc -5' |
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| 22359 | 5' | -53.7 | NC_005045.1 | + | 22030 | 0.7 | 0.50628 |
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Target: 5'- aCGGUGGUCGCauggUCGcuccgcuccuacgcGGUGCG-CCGa -3' miRNA: 3'- aGCCAUCAGCGga--AGC--------------UCAUGCuGGC- -5' |
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| 22359 | 5' | -53.7 | NC_005045.1 | + | 30847 | 0.7 | 0.498895 |
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Target: 5'- gCGGcAGccuccUCGCCgUCGAGUACGACg- -3' miRNA: 3'- aGCCaUC-----AGCGGaAGCUCAUGCUGgc -5' |
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| 22359 | 5' | -53.7 | NC_005045.1 | + | 30815 | 1.09 | 0.001323 |
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Target: 5'- gUCGGUAGUCGCCUUCGAGUACGACCGc -3' miRNA: 3'- -AGCCAUCAGCGGAAGCUCAUGCUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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