Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22363 | 3' | -54 | NC_005045.1 | + | 15377 | 0.66 | 0.700298 |
Target: 5'- uGCUGGAaccgCUgCGGCGGGCcGUGGCg- -3' miRNA: 3'- -CGACUUga--GG-GCUGCUCGaUACCGac -5' |
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22363 | 3' | -54 | NC_005045.1 | + | 39564 | 0.66 | 0.689122 |
Target: 5'- aGCUGuacCUgCCGGCGcugauGGCUGgaGGCUGg -3' miRNA: 3'- -CGACuu-GAgGGCUGC-----UCGAUa-CCGAC- -5' |
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22363 | 3' | -54 | NC_005045.1 | + | 23008 | 0.67 | 0.666605 |
Target: 5'- --aGAGCg-CCGACGAGCUAcaGGCa- -3' miRNA: 3'- cgaCUUGagGGCUGCUCGAUa-CCGac -5' |
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22363 | 3' | -54 | NC_005045.1 | + | 28033 | 0.67 | 0.666605 |
Target: 5'- gGCUGGA---CCGGCGAGUuccUGUGGCg- -3' miRNA: 3'- -CGACUUgagGGCUGCUCG---AUACCGac -5' |
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22363 | 3' | -54 | NC_005045.1 | + | 39498 | 0.67 | 0.621251 |
Target: 5'- cCUGGGCUgCCGACGAGCgcuucaaGCUc -3' miRNA: 3'- cGACUUGAgGGCUGCUCGauac---CGAc -5' |
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22363 | 3' | -54 | NC_005045.1 | + | 22406 | 0.68 | 0.598591 |
Target: 5'- uGCUGAGCgagaaacucggCCUGuACGAGCUGgagucGCUGg -3' miRNA: 3'- -CGACUUGa----------GGGC-UGCUCGAUac---CGAC- -5' |
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22363 | 3' | -54 | NC_005045.1 | + | 19851 | 0.68 | 0.598591 |
Target: 5'- cCUGGACUaCCuCGACGAGUc-UGGCg- -3' miRNA: 3'- cGACUUGA-GG-GCUGCUCGauACCGac -5' |
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22363 | 3' | -54 | NC_005045.1 | + | 25944 | 0.68 | 0.598591 |
Target: 5'- cGCUGAGCUggCCCGGCaGGCgcucgaagGGCa- -3' miRNA: 3'- -CGACUUGA--GGGCUGcUCGaua-----CCGac -5' |
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22363 | 3' | -54 | NC_005045.1 | + | 5071 | 0.68 | 0.57493 |
Target: 5'- cCUGGGCUgCCGACGAcuggacgGCgcggAUGGCg- -3' miRNA: 3'- cGACUUGAgGGCUGCU-------CGa---UACCGac -5' |
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22363 | 3' | -54 | NC_005045.1 | + | 36672 | 0.69 | 0.520712 |
Target: 5'- ----uACUCCCGcUGGGCUGaGGCUGg -3' miRNA: 3'- cgacuUGAGGGCuGCUCGAUaCCGAC- -5' |
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22363 | 3' | -54 | NC_005045.1 | + | 2925 | 0.7 | 0.48851 |
Target: 5'- aCUG-ACUCCUGGCGGGUUcGUGGUa- -3' miRNA: 3'- cGACuUGAGGGCUGCUCGA-UACCGac -5' |
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22363 | 3' | -54 | NC_005045.1 | + | 6947 | 0.7 | 0.48851 |
Target: 5'- cGCUGAACUgguacgggCCGcCGAGC--UGGCUGg -3' miRNA: 3'- -CGACUUGAg-------GGCuGCUCGauACCGAC- -5' |
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22363 | 3' | -54 | NC_005045.1 | + | 34554 | 0.7 | 0.463436 |
Target: 5'- uGCUGAACUCCCGcAUGGGUgacuucguuggGGCc- -3' miRNA: 3'- -CGACUUGAGGGC-UGCUCGaua--------CCGac -5' |
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22363 | 3' | -54 | NC_005045.1 | + | 41849 | 0.83 | 0.064113 |
Target: 5'- gGCUGAGCUggucgcacacCUCGACGAGCUgAUGGCUGc -3' miRNA: 3'- -CGACUUGA----------GGGCUGCUCGA-UACCGAC- -5' |
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22363 | 3' | -54 | NC_005045.1 | + | 33562 | 1.1 | 0.000732 |
Target: 5'- cGCUGAACUCCCGACGAGCUAUGGCUGu -3' miRNA: 3'- -CGACUUGAGGGCUGCUCGAUACCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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