Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22364 | 5' | -58.7 | NC_005045.1 | + | 2648 | 0.66 | 0.430585 |
Target: 5'- cCACGGgcugUUGGUGGcGCCGACGagaucGGUCu -3' miRNA: 3'- aGUGCCa---GACUACC-CGGUUGC-----CCGGu -5' |
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22364 | 5' | -58.7 | NC_005045.1 | + | 3474 | 0.66 | 0.449999 |
Target: 5'- aCACGaaggcacagccGcCUGAUGGGCUGACGGcuaugaugaGCCAc -3' miRNA: 3'- aGUGC-----------CaGACUACCCGGUUGCC---------CGGU- -5' |
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22364 | 5' | -58.7 | NC_005045.1 | + | 3948 | 0.67 | 0.384287 |
Target: 5'- cCACGGaCcgGAUGuGCC-ACGGGCCu -3' miRNA: 3'- aGUGCCaGa-CUACcCGGuUGCCCGGu -5' |
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22364 | 5' | -58.7 | NC_005045.1 | + | 6031 | 0.71 | 0.234138 |
Target: 5'- aCcCGG-CUGGUGgagguaGGUCAACGGGCCGg -3' miRNA: 3'- aGuGCCaGACUAC------CCGGUUGCCCGGU- -5' |
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22364 | 5' | -58.7 | NC_005045.1 | + | 7280 | 0.67 | 0.366714 |
Target: 5'- ---aGGUCaUGcgGGcGCCGuagGCGGGCCGc -3' miRNA: 3'- agugCCAG-ACuaCC-CGGU---UGCCCGGU- -5' |
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22364 | 5' | -58.7 | NC_005045.1 | + | 10346 | 0.7 | 0.259648 |
Target: 5'- cCuuGGUCaGcgGGGUCuuGCGGGCCAu -3' miRNA: 3'- aGugCCAGaCuaCCCGGu-UGCCCGGU- -5' |
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22364 | 5' | -58.7 | NC_005045.1 | + | 27616 | 0.66 | 0.440231 |
Target: 5'- -gAUGGuUCUGAUccagaaGGGCCAGgagaucgcccUGGGCCGu -3' miRNA: 3'- agUGCC-AGACUA------CCCGGUU----------GCCCGGU- -5' |
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22364 | 5' | -58.7 | NC_005045.1 | + | 32940 | 0.66 | 0.440231 |
Target: 5'- cCAgGGUCUGcauGGCC--UGGGCCAc -3' miRNA: 3'- aGUgCCAGACuacCCGGuuGCCCGGU- -5' |
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22364 | 5' | -58.7 | NC_005045.1 | + | 33724 | 1.09 | 0.00033 |
Target: 5'- uUCACGGUCUGAUGGGCCAACGGGCCAg -3' miRNA: 3'- -AGUGCCAGACUACCCGGUUGCCCGGU- -5' |
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22364 | 5' | -58.7 | NC_005045.1 | + | 33936 | 0.73 | 0.155794 |
Target: 5'- cCACGGaacacugccucgCUGAUGGcUCGGCGGGCCAg -3' miRNA: 3'- aGUGCCa-----------GACUACCcGGUUGCCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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