Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22366 | 3' | -61.2 | NC_005045.1 | + | 34356 | 1.1 | 0.000174 |
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Target: 5'- aGGAGCUGACGGGGCAACUGCGGGGCGu -3' miRNA: 3'- -CCUCGACUGCCCCGUUGACGCCCCGC- -5' |
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| 22366 | 3' | -61.2 | NC_005045.1 | + | 35509 | 0.74 | 0.092112 |
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Target: 5'- --cGCUGAcgauaucgcCGGuGGCGGCUGCGGGGUc -3' miRNA: 3'- ccuCGACU---------GCC-CCGUUGACGCCCCGc -5' |
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| 22366 | 3' | -61.2 | NC_005045.1 | + | 23477 | 0.71 | 0.150934 |
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Target: 5'- cGGAGCUacCGGGGcCGGCUgaGCGGGuGCu -3' miRNA: 3'- -CCUCGAcuGCCCC-GUUGA--CGCCC-CGc -5' |
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| 22366 | 3' | -61.2 | NC_005045.1 | + | 32232 | 0.69 | 0.229941 |
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Target: 5'- uGGcGCUGGCGuGGGUgcuCUGCuGGGGUc -3' miRNA: 3'- -CCuCGACUGC-CCCGuu-GACG-CCCCGc -5' |
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| 22366 | 3' | -61.2 | NC_005045.1 | + | 23610 | 0.68 | 0.267597 |
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Target: 5'- -uAGCUGAUGGaGGgugcCAACUGCGGGuCGc -3' miRNA: 3'- ccUCGACUGCC-CC----GUUGACGCCCcGC- -5' |
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| 22366 | 3' | -61.2 | NC_005045.1 | + | 20369 | 0.66 | 0.333177 |
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Target: 5'- aGAGCUGGCGaaaCAGCU-CGGGGUGg -3' miRNA: 3'- cCUCGACUGCcccGUUGAcGCCCCGC- -5' |
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| 22366 | 3' | -61.2 | NC_005045.1 | + | 4747 | 0.66 | 0.365894 |
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Target: 5'- -uGGCgccCGGGGUAGCaGCGGuGGUGg -3' miRNA: 3'- ccUCGacuGCCCCGUUGaCGCC-CCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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