Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22369 | 5' | -52.2 | NC_005045.1 | + | 35721 | 1.13 | 0.000745 |
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Target: 5'- aGAAGGACCAAGAGGUCCGCAUCUUCCg -3' miRNA: 3'- -CUUCCUGGUUCUCCAGGCGUAGAAGG- -5' |
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| 22369 | 5' | -52.2 | NC_005045.1 | + | 34687 | 0.73 | 0.403537 |
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Target: 5'- cGAGGuucaGGCCGAGAacGGUCUGCAUCUcaucagcgaggUCCg -3' miRNA: 3'- -CUUC----CUGGUUCU--CCAGGCGUAGA-----------AGG- -5' |
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| 22369 | 5' | -52.2 | NC_005045.1 | + | 33409 | 0.71 | 0.517083 |
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Target: 5'- --uGGGCCAGGAGGUCgGCcUCa--- -3' miRNA: 3'- cuuCCUGGUUCUCCAGgCGuAGaagg -5' |
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| 22369 | 5' | -52.2 | NC_005045.1 | + | 12337 | 0.68 | 0.720628 |
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Target: 5'- --uGGucGCCGGGAuGUCCGUAUCcUCCc -3' miRNA: 3'- cuuCC--UGGUUCUcCAGGCGUAGaAGG- -5' |
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| 22369 | 5' | -52.2 | NC_005045.1 | + | 4462 | 0.66 | 0.795238 |
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Target: 5'- cGAcGGcccuGCCAAGGugaccGGUCCGCGUCccaCCg -3' miRNA: 3'- -CUuCC----UGGUUCU-----CCAGGCGUAGaa-GG- -5' |
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| 22369 | 5' | -52.2 | NC_005045.1 | + | 13396 | 0.66 | 0.805259 |
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Target: 5'- ---uGACCGuGAGcGUagggCGCAUCUUCCg -3' miRNA: 3'- cuucCUGGUuCUC-CAg---GCGUAGAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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