Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22370 | 5' | -52.2 | NC_005045.1 | + | 6671 | 0.66 | 0.821657 |
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Target: 5'- cGAGAACuGGUGgcaGUGguuCCuCUGGcAGCGg -3' miRNA: 3'- -CUCUUG-CCACa--CACau-GG-GACU-UCGC- -5' |
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| 22370 | 5' | -52.2 | NC_005045.1 | + | 16757 | 0.66 | 0.802056 |
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Target: 5'- cGAGGGCuGG-GUGUGgucGCUCUgGAAGCa -3' miRNA: 3'- -CUCUUG-CCaCACACa--UGGGA-CUUCGc -5' |
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| 22370 | 5' | -52.2 | NC_005045.1 | + | 25850 | 0.68 | 0.716923 |
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Target: 5'- -uGAugGGUGUGagccagcagGUGCCCUucGAGCGc -3' miRNA: 3'- cuCUugCCACACa--------CAUGGGAc-UUCGC- -5' |
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| 22370 | 5' | -52.2 | NC_005045.1 | + | 33853 | 0.68 | 0.705735 |
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Target: 5'- -cGAGCGGUGgaaGUACCCcgGucGCGg -3' miRNA: 3'- cuCUUGCCACacaCAUGGGa-CuuCGC- -5' |
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| 22370 | 5' | -52.2 | NC_005045.1 | + | 30875 | 0.77 | 0.252703 |
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Target: 5'- cGAGAACGGUGUG---GCCCUGGAccgcacGCGg -3' miRNA: 3'- -CUCUUGCCACACacaUGGGACUU------CGC- -5' |
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| 22370 | 5' | -52.2 | NC_005045.1 | + | 36225 | 1.09 | 0.001542 |
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Target: 5'- cGAGAACGGUGUGUGUACCCUGAAGCGc -3' miRNA: 3'- -CUCUUGCCACACACAUGGGACUUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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