Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22371 | 3' | -57.5 | NC_005045.1 | + | 36920 | 0.97 | 0.003543 |
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Target: 5'- cCGCUAA-GUGUCCCUCGUGGCGCCUGu -3' miRNA: 3'- -GCGAUUgCACAGGGAGCACCGCGGAC- -5' |
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| 22371 | 3' | -57.5 | NC_005045.1 | + | 10121 | 0.7 | 0.287475 |
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Target: 5'- gGC-GugGUGUCCCgcagCG-GGCGCCg- -3' miRNA: 3'- gCGaUugCACAGGGa---GCaCCGCGGac -5' |
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| 22371 | 3' | -57.5 | NC_005045.1 | + | 32234 | 0.67 | 0.44097 |
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Target: 5'- gCGCUGGCGUGggugCUCUgcUGGgGUCUGg -3' miRNA: 3'- -GCGAUUGCACa---GGGAgcACCgCGGAC- -5' |
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| 22371 | 3' | -57.5 | NC_005045.1 | + | 9679 | 0.67 | 0.449795 |
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Target: 5'- gGCguGC-UGUCCCUCGUaGGCugccgugGCCUGg -3' miRNA: 3'- gCGauUGcACAGGGAGCA-CCG-------CGGAC- -5' |
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| 22371 | 3' | -57.5 | NC_005045.1 | + | 958 | 0.66 | 0.522532 |
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Target: 5'- cCGCccACGUGUcaauaCCCUCGccagGGCGCUc- -3' miRNA: 3'- -GCGauUGCACA-----GGGAGCa---CCGCGGac -5' |
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| 22371 | 3' | -57.5 | NC_005045.1 | + | 37304 | 0.66 | 0.533159 |
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Target: 5'- gGCUGaguuuggcacucGCGgcaGUCUgUCGUGGCGCgCUa -3' miRNA: 3'- gCGAU------------UGCa--CAGGgAGCACCGCG-GAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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