Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22371 | 5' | -58 | NC_005045.1 | + | 27429 | 0.73 | 0.150908 |
Target: 5'- uGCCGGGGCCg-GCUGAguACauccagGCGGCGg -3' miRNA: 3'- -CGGUCCCGGagCGACU--UGa-----UGCCGUu -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 8625 | 0.72 | 0.193543 |
Target: 5'- cGCCGGGGCCUCgguGCUGG----UGGCAGc -3' miRNA: 3'- -CGGUCCCGGAG---CGACUugauGCCGUU- -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 15363 | 0.67 | 0.377734 |
Target: 5'- gGCCAa-GCCUCcccuGCUGGaaccGCUGCGGCGGg -3' miRNA: 3'- -CGGUccCGGAG----CGACU----UGAUGCCGUU- -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 7634 | 0.67 | 0.386785 |
Target: 5'- cGCCAGGGCUgcCGCUGcuguacAGCgaaaGGCAGc -3' miRNA: 3'- -CGGUCCCGGa-GCGAC------UUGaug-CCGUU- -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 16878 | 0.67 | 0.395978 |
Target: 5'- uGCCAGGGCgCccCGCUGGACgACcuGCAc -3' miRNA: 3'- -CGGUCCCG-Ga-GCGACUUGaUGc-CGUu -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 4214 | 0.66 | 0.464085 |
Target: 5'- gGCCGGGGCCUa-CUGAccggGCUGaaGCGu -3' miRNA: 3'- -CGGUCCCGGAgcGACU----UGAUgcCGUu -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 26424 | 0.66 | 0.474311 |
Target: 5'- aGCguGGGCUacaUCGCuUGGcagcuCUACGGUAAg -3' miRNA: 3'- -CGguCCCGG---AGCG-ACUu----GAUGCCGUU- -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 36953 | 1.07 | 0.000421 |
Target: 5'- uGCCAGGGCCUCGCUGAACUACGGCAAc -3' miRNA: 3'- -CGGUCCCGGAGCGACUUGAUGCCGUU- -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 8539 | 0.66 | 0.453975 |
Target: 5'- gGCgCAGGGCCUCGUc--GCUcagcCGGCGc -3' miRNA: 3'- -CG-GUCCCGGAGCGacuUGAu---GCCGUu -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 23114 | 0.66 | 0.443986 |
Target: 5'- gGCCuGGGCCUgcgcggccUGCUGAcGCUGCuGCu- -3' miRNA: 3'- -CGGuCCCGGA--------GCGACU-UGAUGcCGuu -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 9531 | 0.67 | 0.41478 |
Target: 5'- gGCCAGGGCgacgccCUCGUUGGGCcgugcaucagagUACaGGCGc -3' miRNA: 3'- -CGGUCCCG------GAGCGACUUG------------AUG-CCGUu -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 39190 | 0.67 | 0.404371 |
Target: 5'- cGCgGGuaGGCCguuguagaucgauUCGCgGGACUGCGGCGu -3' miRNA: 3'- -CGgUC--CCGG-------------AGCGaCUUGAUGCCGUu -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 34476 | 0.67 | 0.395978 |
Target: 5'- -aCGGGGCCgaGCUGGuGCUcacGCGGCAc -3' miRNA: 3'- cgGUCCCGGagCGACU-UGA---UGCCGUu -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 23470 | 0.67 | 0.385873 |
Target: 5'- aCCGGGGCCg-GCUGAGCgggugcuUGCGGa-- -3' miRNA: 3'- cGGUCCCGGagCGACUUG-------AUGCCguu -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 11879 | 0.67 | 0.368826 |
Target: 5'- aUCAGGGCCUCGCaGcGCUccaGGUAGc -3' miRNA: 3'- cGGUCCCGGAGCGaCuUGAug-CCGUU- -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 27861 | 0.7 | 0.246363 |
Target: 5'- gGCCAcGGGCUguaGCUGAuAC-ACGGCAGa -3' miRNA: 3'- -CGGU-CCCGGag-CGACU-UGaUGCCGUU- -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 33938 | 0.72 | 0.198883 |
Target: 5'- uGCCAcGGaacacuGCCUCGCUGAugGCU-CGGCGGg -3' miRNA: 3'- -CGGU-CC------CGGAGCGACU--UGAuGCCGUU- -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 8778 | 0.74 | 0.138707 |
Target: 5'- gGCCuGGGUCgaaguacCGCuUGAGCUGCGGCGc -3' miRNA: 3'- -CGGuCCCGGa------GCG-ACUUGAUGCCGUu -5' |
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22371 | 5' | -58 | NC_005045.1 | + | 33748 | 0.77 | 0.080361 |
Target: 5'- gGCCAGGGCCUUGaUGGucgGCGGCAGg -3' miRNA: 3'- -CGGUCCCGGAGCgACUugaUGCCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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