miRNA: 3'- uGCCG---CCGCUG-CUCGACaGCauACGG- -5'
miRNA: 3'- -UGCCGCcgCUGCu----------CGaCAGCAuaCGG- -5'
Results 21 - 22 of 22 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22375 | 3' | -57.3 | NC_005045.1 | + | 18897 | 0.66 | 0.540771 |
|
Target: 5'- cUGGCccaGGUGGCgGAGCUG-CGccUGCCg -3' miRNA: 3'- uGCCG---CCGCUG-CUCGACaGCauACGG- -5' |
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| 22375 | 3' | -57.3 | NC_005045.1 | + | 15546 | 0.66 | 0.562281 |
|
Target: 5'- cAUGGCGuaGGCGuccaucaucauGCcGUCGUcgGCCu -3' miRNA: 3'- -UGCCGCcgCUGCu----------CGaCAGCAuaCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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