Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22378 | 3' | -60.3 | NC_005045.1 | + | 227 | 0.66 | 0.365478 |
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Target: 5'- gGCUCUGGCcCUgGCUGCUGGGu---- -3' miRNA: 3'- aCGAGGCCGuGA-UGGCGACCCcaguu -5' |
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| 22378 | 3' | -60.3 | NC_005045.1 | + | 42290 | 0.66 | 0.365478 |
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Target: 5'- gGCUCUGGCcCUgGCUGCUGGGu---- -3' miRNA: 3'- aCGAGGCCGuGA-UGGCGACCCcaguu -5' |
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| 22378 | 3' | -60.3 | NC_005045.1 | + | 21172 | 0.66 | 0.353489 |
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Target: 5'- gGCUCgGGaguucauccgcgaACUccaccgucagGCCgGCUGGGGUCAAa -3' miRNA: 3'- aCGAGgCCg------------UGA----------UGG-CGACCCCAGUU- -5' |
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| 22378 | 3' | -60.3 | NC_005045.1 | + | 5810 | 0.66 | 0.33197 |
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Target: 5'- cUGCUCaaGGCAUUcCCGCUGGuaUCGAa -3' miRNA: 3'- -ACGAGg-CCGUGAuGGCGACCccAGUU- -5' |
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| 22378 | 3' | -60.3 | NC_005045.1 | + | 9890 | 0.67 | 0.293339 |
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Target: 5'- cGCacCCaGGCGCggGCCGCcuucGGGGUCGGg -3' miRNA: 3'- aCGa-GG-CCGUGa-UGGCGa---CCCCAGUU- -5' |
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| 22378 | 3' | -60.3 | NC_005045.1 | + | 18751 | 0.68 | 0.232196 |
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Target: 5'- cGCUCCGGCcaggGCcgccccaggucauUGCCGCcgaGGGGUCc- -3' miRNA: 3'- aCGAGGCCG----UG-------------AUGGCGa--CCCCAGuu -5' |
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| 22378 | 3' | -60.3 | NC_005045.1 | + | 18225 | 0.69 | 0.220868 |
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Target: 5'- aGCUCCGGCACU--UGCUGcGGaUCAu -3' miRNA: 3'- aCGAGGCCGUGAugGCGAC-CCcAGUu -5' |
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| 22378 | 3' | -60.3 | NC_005045.1 | + | 9403 | 0.74 | 0.096452 |
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Target: 5'- gGUUCCauuGCACUGCCGaCcGGGGUCAAg -3' miRNA: 3'- aCGAGGc--CGUGAUGGC-GaCCCCAGUU- -5' |
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| 22378 | 3' | -60.3 | NC_005045.1 | + | 32225 | 0.75 | 0.081222 |
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Target: 5'- gUGCUCuUGGCGCUGgcgugggugcuCUGCUGGGGUCu- -3' miRNA: 3'- -ACGAG-GCCGUGAU-----------GGCGACCCCAGuu -5' |
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| 22378 | 3' | -60.3 | NC_005045.1 | + | 40615 | 1.06 | 0.000319 |
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Target: 5'- cUGCUCCGGCACUACCGCUGGGGUCAAg -3' miRNA: 3'- -ACGAGGCCGUGAUGGCGACCCCAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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