miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22379 5' -57.7 NC_005045.1 + 41295 1.06 0.00051
Target:  5'- gAUCCGCGCUGUAGCGGCCUAUCAGGAc -3'
miRNA:   3'- -UAGGCGCGACAUCGCCGGAUAGUCCU- -5'
22379 5' -57.7 NC_005045.1 + 9270 0.7 0.270646
Target:  5'- gAUCCGCaccGUUGaGGCGGCCUAgguGGAu -3'
miRNA:   3'- -UAGGCG---CGACaUCGCCGGAUaguCCU- -5'
22379 5' -57.7 NC_005045.1 + 23873 0.69 0.315377
Target:  5'- gGUCCGCGUguugGUugaagacgccGGCaGCCUGcuUCAGGAc -3'
miRNA:   3'- -UAGGCGCGa---CA----------UCGcCGGAU--AGUCCU- -5'
22379 5' -57.7 NC_005045.1 + 30734 0.68 0.348179
Target:  5'- --gCGCGCUGUcgcGGCGGCUgaaCAGGu -3'
miRNA:   3'- uagGCGCGACA---UCGCCGGauaGUCCu -5'
22379 5' -57.7 NC_005045.1 + 33131 0.68 0.36547
Target:  5'- -aCCGuCGCUGUAGCgcaGGCgguUCAGGGu -3'
miRNA:   3'- uaGGC-GCGACAUCG---CCGgauAGUCCU- -5'
22379 5' -57.7 NC_005045.1 + 13886 0.66 0.440324
Target:  5'- -gCCGCGCUGccguccgGGUGGUCcacgaacGUCAGGGu -3'
miRNA:   3'- uaGGCGCGACa------UCGCCGGa------UAGUCCU- -5'
22379 5' -57.7 NC_005045.1 + 2908 0.66 0.491295
Target:  5'- -gCUGCGUUGcAGCGGCCcgcCGGGu -3'
miRNA:   3'- uaGGCGCGACaUCGCCGGauaGUCCu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.