Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22380 | 3' | -57.1 | NC_005045.1 | + | 34875 | 0.66 | 0.509668 |
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Target: 5'- cGCCCGC--GCCaCCUuguuCAAGGUGAGg -3' miRNA: 3'- cUGGGCGagUGGcGGAu---GUUCCACUU- -5' |
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| 22380 | 3' | -57.1 | NC_005045.1 | + | 34789 | 0.66 | 0.482189 |
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Target: 5'- cGGCCUGCUUACCuGCgUGCAggaagcgauccaugaAGGUGu- -3' miRNA: 3'- -CUGGGCGAGUGG-CGgAUGU---------------UCCACuu -5' |
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| 22380 | 3' | -57.1 | NC_005045.1 | + | 29798 | 0.67 | 0.427566 |
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Target: 5'- cACCCGCUguuggaagucugCAUCgGCCcGCAAGGUGGc -3' miRNA: 3'- cUGGGCGA------------GUGG-CGGaUGUUCCACUu -5' |
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| 22380 | 3' | -57.1 | NC_005045.1 | + | 10454 | 0.67 | 0.417856 |
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Target: 5'- gGACCauggcgcggCGCUCAUCGUacACGAGGUGGGa -3' miRNA: 3'- -CUGG---------GCGAGUGGCGgaUGUUCCACUU- -5' |
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| 22380 | 3' | -57.1 | NC_005045.1 | + | 6520 | 0.68 | 0.371391 |
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Target: 5'- cGCCCGCUUGCCGUCgaUGCAcGG-GAAg -3' miRNA: 3'- cUGGGCGAGUGGCGG--AUGUuCCaCUU- -5' |
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| 22380 | 3' | -57.1 | NC_005045.1 | + | 5845 | 0.69 | 0.336839 |
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Target: 5'- cGGCCCGCUguCCGCCaaggacuuCGAGGUa-- -3' miRNA: 3'- -CUGGGCGAguGGCGGau------GUUCCAcuu -5' |
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| 22380 | 3' | -57.1 | NC_005045.1 | + | 35194 | 0.7 | 0.282159 |
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Target: 5'- uAUCCGCUCACCGCgCUGaugauGGGUGc- -3' miRNA: 3'- cUGGGCGAGUGGCG-GAUgu---UCCACuu -5' |
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| 22380 | 3' | -57.1 | NC_005045.1 | + | 8247 | 0.73 | 0.187405 |
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Target: 5'- aGACCCGCaggCGCUGCgcgaacugaucaagCUGCGAGGUGGu -3' miRNA: 3'- -CUGGGCGa--GUGGCG--------------GAUGUUCCACUu -5' |
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| 22380 | 3' | -57.1 | NC_005045.1 | + | 41551 | 1.06 | 0.000663 |
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Target: 5'- gGACCCGCUCACCGCCUACAAGGUGAAg -3' miRNA: 3'- -CUGGGCGAGUGGCGGAUGUUCCACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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