Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22394 | 3' | -49.7 | NC_005056.1 | + | 10150 | 0.66 | 0.874979 |
Target: 5'- uGCUCAAGGUcAGuGACaGUCAg--GCCa -3' miRNA: 3'- -CGAGUUCCGuUUuCUG-CAGUaggCGG- -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 22675 | 0.67 | 0.848602 |
Target: 5'- aCUCAgaAGGgAAAuuuauaACGUCAcUCCGCCu -3' miRNA: 3'- cGAGU--UCCgUUUuc----UGCAGU-AGGCGG- -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 6330 | 0.67 | 0.839271 |
Target: 5'- uUUCAccGUc---GAUGUCGUCCGCCg -3' miRNA: 3'- cGAGUucCGuuuuCUGCAGUAGGCGG- -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 7029 | 0.67 | 0.829687 |
Target: 5'- uCUCGAcaugcuGGCAguguccgaAGGGACGgcgaaUCAUCCGCUg -3' miRNA: 3'- cGAGUU------CCGU--------UUUCUGC-----AGUAGGCGG- -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 29088 | 0.68 | 0.798504 |
Target: 5'- aGCUCAucGGGCGucAGAuuuuCGUUgaccauaAUCCGCUg -3' miRNA: 3'- -CGAGU--UCCGUuuUCU----GCAG-------UAGGCGG- -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 18288 | 0.68 | 0.778411 |
Target: 5'- uGCUC-AGGCGAAAGuGCuggaAUCCGCa -3' miRNA: 3'- -CGAGuUCCGUUUUC-UGcag-UAGGCGg -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 6024 | 0.68 | 0.756578 |
Target: 5'- gGCUCccguguccgguGAGGguAcGGugGUCAUcCCGCa -3' miRNA: 3'- -CGAG-----------UUCCguUuUCugCAGUA-GGCGg -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 12200 | 0.69 | 0.745433 |
Target: 5'- gGCUCAGGGCGuucgcAGACcugccgcaugGUCAUCauCGUCa -3' miRNA: 3'- -CGAGUUCCGUuu---UCUG----------CAGUAG--GCGG- -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 7399 | 0.69 | 0.734156 |
Target: 5'- cGCUCAAuGCGucccgcccGGAUGUCAUCCaGCg -3' miRNA: 3'- -CGAGUUcCGUuu------UCUGCAGUAGG-CGg -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 22104 | 0.69 | 0.71126 |
Target: 5'- cCUCAA-GCucuAAGGACGUCGUgaagCCGCUa -3' miRNA: 3'- cGAGUUcCGu--UUUCUGCAGUA----GGCGG- -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 278 | 0.7 | 0.688004 |
Target: 5'- gGCUC--GGUc-AGGAgGUCAUCCGCUu -3' miRNA: 3'- -CGAGuuCCGuuUUCUgCAGUAGGCGG- -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 1442 | 0.7 | 0.676276 |
Target: 5'- aGC-CAcGGCGGAuGACGUCuUuuGCCu -3' miRNA: 3'- -CGaGUuCCGUUUuCUGCAGuAggCGG- -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 17077 | 0.7 | 0.652687 |
Target: 5'- -aUCAGGGCGuugcAAAGucCGUCAcgCUGCCa -3' miRNA: 3'- cgAGUUCCGU----UUUCu-GCAGUa-GGCGG- -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 21867 | 0.72 | 0.558424 |
Target: 5'- uGgUCAGGGCAAAA-ACGUUGUCaGCCu -3' miRNA: 3'- -CgAGUUCCGUUUUcUGCAGUAGgCGG- -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 18044 | 0.73 | 0.479245 |
Target: 5'- uGCUCAGGGCuguuGACcaggCAUcCCGCCc -3' miRNA: 3'- -CGAGUUCCGuuuuCUGca--GUA-GGCGG- -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 26870 | 0.74 | 0.416006 |
Target: 5'- gGCgggCAAuGGCAGAAuaugccugccucGugGUCAUCUGCCc -3' miRNA: 3'- -CGa--GUU-CCGUUUU------------CugCAGUAGGCGG- -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 18689 | 0.75 | 0.367385 |
Target: 5'- uGCUCGgcguGGCAAAAGAC----UCCGCCg -3' miRNA: 3'- -CGAGUu---CCGUUUUCUGcaguAGGCGG- -5' |
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22394 | 3' | -49.7 | NC_005056.1 | + | 1506 | 1.16 | 0.000686 |
Target: 5'- uGCUCAAGGCAAAAGACGUCAUCCGCCg -3' miRNA: 3'- -CGAGUUCCGUUUUCUGCAGUAGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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