Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22394 | 5' | -52.8 | NC_005056.1 | + | 2091 | 0.66 | 0.725479 |
Target: 5'- gUGGAAaucGACGUCAGUcauaacGCCcuuGCCGGa -3' miRNA: 3'- gACCUUauaCUGCAGUCG------CGG---UGGCU- -5' |
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22394 | 5' | -52.8 | NC_005056.1 | + | 10637 | 0.66 | 0.714219 |
Target: 5'- -cGGGcgGUGAUG-CuggccuGUGCCGCCGGu -3' miRNA: 3'- gaCCUuaUACUGCaGu-----CGCGGUGGCU- -5' |
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22394 | 5' | -52.8 | NC_005056.1 | + | 10988 | 0.66 | 0.70287 |
Target: 5'- aUGGugag-GACGUCAG-GCCggugGCCGAc -3' miRNA: 3'- gACCuuauaCUGCAGUCgCGG----UGGCU- -5' |
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22394 | 5' | -52.8 | NC_005056.1 | + | 1404 | 0.67 | 0.668411 |
Target: 5'- gCUGG----UGAUGUCGGUGgCGCUGAc -3' miRNA: 3'- -GACCuuauACUGCAGUCGCgGUGGCU- -5' |
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22394 | 5' | -52.8 | NC_005056.1 | + | 7084 | 0.67 | 0.621989 |
Target: 5'- -cGGGGcUAUGACGUgAuaGUCACCGGa -3' miRNA: 3'- gaCCUU-AUACUGCAgUcgCGGUGGCU- -5' |
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22394 | 5' | -52.8 | NC_005056.1 | + | 18567 | 0.69 | 0.508027 |
Target: 5'- -cGGAAcUAUGGCcucaGUgGGCGCUGCCGGu -3' miRNA: 3'- gaCCUU-AUACUG----CAgUCGCGGUGGCU- -5' |
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22394 | 5' | -52.8 | NC_005056.1 | + | 19710 | 0.7 | 0.486191 |
Target: 5'- aUGGcaccgAUGACGcCAgucagcgccaguGCGCCACCGAc -3' miRNA: 3'- gACCuua--UACUGCaGU------------CGCGGUGGCU- -5' |
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22394 | 5' | -52.8 | NC_005056.1 | + | 4909 | 0.71 | 0.433691 |
Target: 5'- uCUGGcugaugAUGACGUCAGCgGCCAgCa- -3' miRNA: 3'- -GACCuua---UACUGCAGUCG-CGGUgGcu -5' |
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22394 | 5' | -52.8 | NC_005056.1 | + | 4378 | 0.71 | 0.394149 |
Target: 5'- -cGGc---UGACGcUCGGUGCCACCGGc -3' miRNA: 3'- gaCCuuauACUGC-AGUCGCGGUGGCU- -5' |
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22394 | 5' | -52.8 | NC_005056.1 | + | 2117 | 0.72 | 0.339396 |
Target: 5'- -gGGGAc---GCGcCAGCGCCGCCGAa -3' miRNA: 3'- gaCCUUauacUGCaGUCGCGGUGGCU- -5' |
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22394 | 5' | -52.8 | NC_005056.1 | + | 1472 | 1.09 | 0.000963 |
Target: 5'- uCUGGAAUAUGACGUCAGCGCCACCGAc -3' miRNA: 3'- -GACCUUAUACUGCAGUCGCGGUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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