Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22397 | 3' | -54.1 | NC_005056.1 | + | 910 | 0.7 | 0.371352 |
|
Target: 5'- aGCGUUACAGCAcuACCGguaaagUCAUCAGAc -3' miRNA: 3'- -UGCGGUGUCGUacUGGC------AGUAGUCUu -5' |
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| 22397 | 3' | -54.1 | NC_005056.1 | + | 1729 | 0.66 | 0.596194 |
|
Target: 5'- uCGCCACGGUGUGACgGgUCGUaaccaaucCAGAc -3' miRNA: 3'- uGCGGUGUCGUACUGgC-AGUA--------GUCUu -5' |
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| 22397 | 3' | -54.1 | NC_005056.1 | + | 4058 | 0.72 | 0.271373 |
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Target: 5'- gACGCCGguCAGCAgu-UCGUCAUCAGGg -3' miRNA: 3'- -UGCGGU--GUCGUacuGGCAGUAGUCUu -5' |
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| 22397 | 3' | -54.1 | NC_005056.1 | + | 5458 | 1.06 | 0.000896 |
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Target: 5'- gACGCCACAGCAUGACCGUCAUCAGAAu -3' miRNA: 3'- -UGCGGUGUCGUACUGGCAGUAGUCUU- -5' |
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| 22397 | 3' | -54.1 | NC_005056.1 | + | 5743 | 0.66 | 0.584536 |
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Target: 5'- aGCGCCcgGCGGCAUcACCagcGUCAcgCAGAAa -3' miRNA: 3'- -UGCGG--UGUCGUAcUGG---CAGUa-GUCUU- -5' |
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| 22397 | 3' | -54.1 | NC_005056.1 | + | 17911 | 0.66 | 0.572923 |
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Target: 5'- gAUGUgGCAGUGauauuucacUGGCCGcCAUCAGAAc -3' miRNA: 3'- -UGCGgUGUCGU---------ACUGGCaGUAGUCUU- -5' |
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| 22397 | 3' | -54.1 | NC_005056.1 | + | 20359 | 0.67 | 0.538453 |
|
Target: 5'- uGCGCCggggacACAGCuUGACCGcCAguuaCAGGAu -3' miRNA: 3'- -UGCGG------UGUCGuACUGGCaGUa---GUCUU- -5' |
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| 22397 | 3' | -54.1 | NC_005056.1 | + | 20721 | 0.68 | 0.461242 |
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Target: 5'- -aGCCugAGCGUGGCa-UCGUCAGc- -3' miRNA: 3'- ugCGGugUCGUACUGgcAGUAGUCuu -5' |
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| 22397 | 3' | -54.1 | NC_005056.1 | + | 21571 | 0.69 | 0.399975 |
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Target: 5'- cAC-CCGCAGCGacggcGACCGUCAUCAu-- -3' miRNA: 3'- -UGcGGUGUCGUa----CUGGCAGUAGUcuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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