Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22397 | 5' | -58 | NC_005056.1 | + | 16889 | 0.66 | 0.451691 |
Target: 5'- cGCUgaaCCAGCAgGUCG--CAUCCACGa -3' miRNA: 3'- aCGA---GGUCGUgCGGCaaGUGGGUGCc -5' |
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22397 | 5' | -58 | NC_005056.1 | + | 1369 | 0.66 | 0.431906 |
Target: 5'- aGCgaggaagCCAGcCACGCCGaccUCGCCUggGCGa -3' miRNA: 3'- aCGa------GGUC-GUGCGGCa--AGUGGG--UGCc -5' |
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22397 | 5' | -58 | NC_005056.1 | + | 21282 | 0.66 | 0.422205 |
Target: 5'- --aUCCGGCGCGCCccgguGUUCaACCUcaucaACGGu -3' miRNA: 3'- acgAGGUCGUGCGG-----CAAG-UGGG-----UGCC- -5' |
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22397 | 5' | -58 | NC_005056.1 | + | 15883 | 0.66 | 0.412637 |
Target: 5'- aGCaCCuGCACGCCGUgaugauagUCACUCAUc- -3' miRNA: 3'- aCGaGGuCGUGCGGCA--------AGUGGGUGcc -5' |
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22397 | 5' | -58 | NC_005056.1 | + | 17819 | 0.66 | 0.403203 |
Target: 5'- cUGCUCgguCAGCAuuggUGCCG-UCAUgCGCGGc -3' miRNA: 3'- -ACGAG---GUCGU----GCGGCaAGUGgGUGCC- -5' |
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22397 | 5' | -58 | NC_005056.1 | + | 5697 | 0.68 | 0.308893 |
Target: 5'- aUGCcgCCGGUGCGCUGgcUCACCUGCa- -3' miRNA: 3'- -ACGa-GGUCGUGCGGCa-AGUGGGUGcc -5' |
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22397 | 5' | -58 | NC_005056.1 | + | 9670 | 0.69 | 0.271932 |
Target: 5'- gGCcagaaUCAGCAC-CUGUUCcCCCACGGa -3' miRNA: 3'- aCGa----GGUCGUGcGGCAAGuGGGUGCC- -5' |
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22397 | 5' | -58 | NC_005056.1 | + | 10135 | 0.7 | 0.226266 |
Target: 5'- gGCgauaUCCugcGCACGCCGgUCGgCUCACGGg -3' miRNA: 3'- aCG----AGGu--CGUGCGGCaAGU-GGGUGCC- -5' |
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22397 | 5' | -58 | NC_005056.1 | + | 1582 | 0.71 | 0.203202 |
Target: 5'- gUGUUCCAGCucguGCGCuCGUUCuauCCCGCc- -3' miRNA: 3'- -ACGAGGUCG----UGCG-GCAAGu--GGGUGcc -5' |
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22397 | 5' | -58 | NC_005056.1 | + | 17548 | 0.81 | 0.041405 |
Target: 5'- uUGCUCCAGCcggucgACGCCGUUCACCUucucaaucauguugAUGGu -3' miRNA: 3'- -ACGAGGUCG------UGCGGCAAGUGGG--------------UGCC- -5' |
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22397 | 5' | -58 | NC_005056.1 | + | 5409 | 1.09 | 0.000274 |
Target: 5'- cUGCUCCAGCACGCCGUUCACCCACGGc -3' miRNA: 3'- -ACGAGGUCGUGCGGCAAGUGGGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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