miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22398 3' -55.9 NC_005056.1 + 5630 1.12 0.000291
Target:  5'- aGCAGGCGGGUAUCCACAGACUCACCGg -3'
miRNA:   3'- -CGUCCGCCCAUAGGUGUCUGAGUGGC- -5'
22398 3' -55.9 NC_005056.1 + 1664 0.75 0.149168
Target:  5'- uCAGGCGGaauccAUCCGCAaACUCACCGa -3'
miRNA:   3'- cGUCCGCCca---UAGGUGUcUGAGUGGC- -5'
22398 3' -55.9 NC_005056.1 + 16013 0.71 0.287199
Target:  5'- cGCuGGCGGcGUcgUUGCAGGCuaUCGCCGa -3'
miRNA:   3'- -CGuCCGCC-CAuaGGUGUCUG--AGUGGC- -5'
22398 3' -55.9 NC_005056.1 + 11464 0.67 0.486808
Target:  5'- gGCAGGUguccGGGU-UCCACAG-UUCACg- -3'
miRNA:   3'- -CGUCCG----CCCAuAGGUGUCuGAGUGgc -5'
22398 3' -55.9 NC_005056.1 + 20083 0.66 0.562986
Target:  5'- gGCAacGGUucaGGGUGUCCugGGugGCUUugACCGg -3'
miRNA:   3'- -CGU--CCG---CCCAUAGGugUC--UGAG--UGGC- -5'
22398 3' -55.9 NC_005056.1 + 2228 0.66 0.562986
Target:  5'- aGguGGCaucaGGUAUggccUCACAGAgUCACCu -3'
miRNA:   3'- -CguCCGc---CCAUA----GGUGUCUgAGUGGc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.