Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22398 | 5' | -61.5 | NC_005056.1 | + | 9103 | 0.66 | 0.279111 |
Target: 5'- uGGCGCGUaggGUGuGCCGUccgGGGCuuucuguGCCAUCa -3' miRNA: 3'- -CCGCGCG---UAC-CGGCG---UUCG-------CGGUGGa -5' |
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22398 | 5' | -61.5 | NC_005056.1 | + | 11205 | 0.66 | 0.265892 |
Target: 5'- aGGCGUGCAgcgugucgagcUGGCCGCA----CCACUg -3' miRNA: 3'- -CCGCGCGU-----------ACCGGCGUucgcGGUGGa -5' |
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22398 | 5' | -61.5 | NC_005056.1 | + | 8647 | 0.67 | 0.233527 |
Target: 5'- cGGUGCugGCGUGGCUGCGGGaaaaucaGCCcgacaucaugACCa -3' miRNA: 3'- -CCGCG--CGUACCGGCGUUCg------CGG----------UGGa -5' |
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22398 | 5' | -61.5 | NC_005056.1 | + | 4615 | 0.67 | 0.221538 |
Target: 5'- cGGCGCGCGUGaugaGCAAG-GUCACUg -3' miRNA: 3'- -CCGCGCGUACcgg-CGUUCgCGGUGGa -5' |
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22398 | 5' | -61.5 | NC_005056.1 | + | 8040 | 0.67 | 0.210077 |
Target: 5'- aGGCGCGCAggcUGGCguuuucaugcuuCGCcAGaaacagcccCGCCACCg -3' miRNA: 3'- -CCGCGCGU---ACCG------------GCGuUC---------GCGGUGGa -5' |
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22398 | 5' | -61.5 | NC_005056.1 | + | 8244 | 0.68 | 0.178724 |
Target: 5'- uGUGCGCc-GGuuGCAccAGCGCCcgGCCUg -3' miRNA: 3'- cCGCGCGuaCCggCGU--UCGCGG--UGGA- -5' |
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22398 | 5' | -61.5 | NC_005056.1 | + | 12687 | 0.7 | 0.143415 |
Target: 5'- cGGCuuuCGuUAUGGCCGCGAuUGCCGCCc -3' miRNA: 3'- -CCGc--GC-GUACCGGCGUUcGCGGUGGa -5' |
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22398 | 5' | -61.5 | NC_005056.1 | + | 1289 | 0.72 | 0.090731 |
Target: 5'- gGGCGUGCAUGGUCGCccaggcgaggucGGCGUgGCUg -3' miRNA: 3'- -CCGCGCGUACCGGCGu-----------UCGCGgUGGa -5' |
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22398 | 5' | -61.5 | NC_005056.1 | + | 20704 | 0.73 | 0.081346 |
Target: 5'- gGGCGgGCGUGGCCGUugcuGGaUGgCACCg -3' miRNA: 3'- -CCGCgCGUACCGGCGu---UC-GCgGUGGa -5' |
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22398 | 5' | -61.5 | NC_005056.1 | + | 27770 | 0.73 | 0.079034 |
Target: 5'- -aCGCGuCGaGGCUGCAcGCGCCGCCg -3' miRNA: 3'- ccGCGC-GUaCCGGCGUuCGCGGUGGa -5' |
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22398 | 5' | -61.5 | NC_005056.1 | + | 21903 | 0.73 | 0.072471 |
Target: 5'- cGGCGCGCAUGGCCu---GCGCUuucguggcauauACCg -3' miRNA: 3'- -CCGCGCGUACCGGcguuCGCGG------------UGGa -5' |
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22398 | 5' | -61.5 | NC_005056.1 | + | 5865 | 0.74 | 0.06268 |
Target: 5'- gGGCGCGgGUGGCCGCuuuGGGCuGCgGCUc -3' miRNA: 3'- -CCGCGCgUACCGGCG---UUCG-CGgUGGa -5' |
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22398 | 5' | -61.5 | NC_005056.1 | + | 5596 | 1.08 | 0.000124 |
Target: 5'- gGGCGCGCAUGGCCGCAAGCGCCACCUc -3' miRNA: 3'- -CCGCGCGUACCGGCGUUCGCGGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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