Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
224 | 3' | -54.9 | AC_000008.1 | + | 10379 | 0.66 | 0.600126 |
Target: 5'- gGUGGCcgGGGCuccgggGGCGagaUCUUCCAACauaagGCg -3' miRNA: 3'- gUACUG--CCCG------CCGC---AGAAGGUUGa----CG- -5' |
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224 | 3' | -54.9 | AC_000008.1 | + | 2267 | 0.66 | 0.600126 |
Target: 5'- --cGGCGGGCaGGUuUUggCCAGCUGg -3' miRNA: 3'- guaCUGCCCG-CCGcAGaaGGUUGACg -5' |
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224 | 3' | -54.9 | AC_000008.1 | + | 9737 | 0.69 | 0.458475 |
Target: 5'- cCGUGGCGGGCGGCag---CgGGCgGCg -3' miRNA: 3'- -GUACUGCCCGCCGcagaaGgUUGaCG- -5' |
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224 | 3' | -54.9 | AC_000008.1 | + | 6518 | 0.69 | 0.418445 |
Target: 5'- aAUGGCGGuaGGgGg--UCUAGCUGCg -3' miRNA: 3'- gUACUGCCcgCCgCagaAGGUUGACG- -5' |
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224 | 3' | -54.9 | AC_000008.1 | + | 12332 | 0.69 | 0.408774 |
Target: 5'- gGUG-CGGGCGGCG-CUgcagagCCAGCcGUc -3' miRNA: 3'- gUACuGCCCGCCGCaGAa-----GGUUGaCG- -5' |
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224 | 3' | -54.9 | AC_000008.1 | + | 8188 | 0.7 | 0.389855 |
Target: 5'- -cUGGCGGGUuuGGCuggugGUCUUCUAcuucgGCUGCu -3' miRNA: 3'- guACUGCCCG--CCG-----CAGAAGGU-----UGACG- -5' |
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224 | 3' | -54.9 | AC_000008.1 | + | 17745 | 0.7 | 0.371519 |
Target: 5'- -cUGACGGGCGGCaugcGUCgugcgcaccaCCGGCgGCg -3' miRNA: 3'- guACUGCCCGCCG----CAGaa--------GGUUGaCG- -5' |
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224 | 3' | -54.9 | AC_000008.1 | + | 26348 | 1.13 | 0.000347 |
Target: 5'- aCAUGACGGGCGGCGUCUUCCAACUGCg -3' miRNA: 3'- -GUACUGCCCGCCGCAGAAGGUUGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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